The reagents in the Qtracker® 655 Cell Labeling Kit use a custom targeting peptide to deliver red-fluorescent Qdot® 655 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.
• Qtracker® 655 label has Ex/Em (405-615/655) nm • Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals • Provide intense, stable fluorescence that can be traced through several generations • Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission • Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays
The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.
Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.
Long-Lasting, Targeted Signal Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.
Monitor the Signal on Multiple Platforms Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.
Minimal Impact on Live Cells The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.
Useful in a Variety of Cell Tracing Studies Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.
For Research Use Only. Not for use in diagnostic procedures.
Contains 100 µl Qtracker™ nanocrystals, Component A (2 µM in 50 mM borate buffer, pH 8.3) and 100 µl Qtracker™ carrier,Component B (phosphate buffered saline, pH 7.2). Store at 2–6°C. Do not freeze. Stable for at least 6 months.