Quant-iT™ microRNA Reagent

Catalog number: Q32883

Invitrogen™  Related applications: Nucleic Acid Quantitation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Quant-iT™ microRNA Reagent allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng following the supplied protocol.

Features of Quant-iT™ microRNA Reagent:

• Accurately detects as little as 0.5 ng miRNA in a microplate well
• Core dynamic range of 5–500 ng/mL
• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL
• Approximate fluorescence excitation/emission maxima of 500/525 nm

Using the protocol provided, this concentrated assay reagent provides sufficient material to perform at least 2000 assays in a 96-well microplate format. The assay is performed at room temperature, and the signal is stable for approximately 3 hours. Accurate results can be obtained for initial sample concentrations ranging from 50 ng/mL–100 µg/mL.

Which product to choose for microRNA Quantitation?

• For 1–20 samples: use the Qubit® microRNA Assay Kit with the Qubit® Fluorometer
• For 20–2,000 samples: use the Quant-iT™ microRNA Assay Kit with microplate reader
• For >2,000 samples: use this Quant-iT™ microRNA Reagent with microplate reader

Discover the Qubit® Fluorometer
The Qubit® 2.0 Fluorometer is Life Technologies’ second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit® quantitation assays as well as the assays you create with the new MyQubit feature.

Introducing MyQubit
New firmware called "MyQubit" allows you to create applications in minutes using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. All new Qubit® 2.0 instruments are pre-loaded with the new MyQubit firmware, and existing owners can download the firmware here: V3.10 firmware download. Any reagent or assay that is spectrally compatible with the Qubit® hardware can be adapted for use with the Qubit® 2.0 Fluorometer. Additionally we have developed assays that are ready to download, including glucose and cholesterol assays for research and drug discovery applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): RNA, FFPE & Fixed Samples
For Use With (Equipment): Microplate Reader, Fluorometer
Product Size: 1 mL
Sample Type (Specific): mRNA, Tissue (FFPE), Total RNA (Bacterial), mRNA (Bacterial), Total RNA (Yeast), RNA (<40 bases), Total RNA, miRNA

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

Documents

Manuals & protocols

Recommended products