New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.
Invitrogen™

Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Catalog number: Q33212
Invitrogen™

Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Catalog number: Q33212
Quantify protein quickly and accurately with two unique Qubit protein quantification kits: the Qubit Protein Assay, which quantifies 12.5 µg/mL to 5 mg/mL of protein, and the Qubit Protein BR Assay Kit, which quantifies 0.1 to 20 mg/mL of protein. Both assays are compatible with many common contaminants, reducing reagents, salts, and nucleic acids. The Protein BR Assay Kit is also tolerant of detergents.
 
Catalog Number
Q33212
Unit Size
T
Assay
Protein Quantification Assay
Quantitation Range
0.25 to 5 µg
Price (USD)
321.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Qubit Fluorometer, Microplate Reader
Quantitation Range0.25 to 5 µg
AssayProtein Quantification Assay
Product LineQuant-iT, Qubit
Quantity500 reactions
Shipping ConditionRoom Temperature
Detection MethodFluorescent
Unit SizeT
Catalog Number
Unit Size
T
Assay
Protein Quantification Assay
Quantitation Range
0.25 to 5 µg
Price (USD)
106.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Qubit Fluorometer, Microplate Reader
Quantitation Range0.25 to 5 µg
AssayProtein Quantification Assay
Product LineQuant-iT, Qubit
Quantity100 reactions
Shipping ConditionRoom Temperature
Detection MethodFluorescent
Unit SizeT
Catalog Number
Unit Size
100 assays
Assay
Protein Quantification, broad range
Quantitation Range
0.1 to 20 mg/mL
Price (USD)
125.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Qubit 4 Fluorometer
Quantitation Range0.1 to 20 mg/mL
AssayProtein Quantification, broad range
Product LineQubit
Shipping ConditionWet Ice
Detection MethodFluorescent
Unit Size100 assays
Catalog Number
Unit Size
500 assays
Assay
Protein Quantification Assay, broad range
Quantitation Range
0.1 to 20 mg/mL
Price (USD)
250.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Qubit 4 Fluorometer
Quantitation Range0.1 to 20 mg/mL
AssayProtein Quantification Assay, broad range
Product LineQubit
Shipping ConditionWet Ice
Detection MethodFluorescent
Unit Size500 assays
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeAssayQuantitation RangePrice (USD)AvailabilityQuantity
Q33212Full specifications
TProtein Quantification Assay0.25 to 5 µg
321.00
Your Price:
***
For Use With (Equipment)Qubit Fluorometer, Microplate Reader
Quantitation Range0.25 to 5 µg
AssayProtein Quantification Assay
Product LineQuant-iT, Qubit
Quantity500 reactions
Shipping ConditionRoom Temperature
Detection MethodFluorescent
Unit SizeT
Q33211Full specifications
TProtein Quantification Assay0.25 to 5 µg
106.00
Your Price:
***
For Use With (Equipment)Qubit Fluorometer, Microplate Reader
Quantitation Range0.25 to 5 µg
AssayProtein Quantification Assay
Product LineQuant-iT, Qubit
Quantity100 reactions
Shipping ConditionRoom Temperature
Detection MethodFluorescent
Unit SizeT
A50668Full specifications
100 assaysProtein Quantification, broad range0.1 to 20 mg/mL
125.00
Your Price:
***
For Use With (Equipment)Qubit 4 Fluorometer
Quantitation Range0.1 to 20 mg/mL
AssayProtein Quantification, broad range
Product LineQubit
Shipping ConditionWet Ice
Detection MethodFluorescent
Unit Size100 assays
A50669Full specifications
500 assaysProtein Quantification Assay, broad range0.1 to 20 mg/mL
250.00
Your Price:
***
For Use With (Equipment)Qubit 4 Fluorometer
Quantitation Range0.1 to 20 mg/mL
AssayProtein Quantification Assay, broad range
Product LineQubit
Shipping ConditionWet Ice
Detection MethodFluorescent
Unit Size500 assays
Showing 4 of 4
Qubit Protein Assay Kit
The Qubit Protein Assay Kit is designed specifically for use with any Qubit fluorometer. While using between 1 and 20 µL of sample, this assay can quantify samples ranging from 12.5 µg⁄mL to 5 mg⁄mL with low protein-to-protein variation.

The Qubit Protein Assay Kit is highly selective for proteins and is also highly accurate in the presence of reducing reagents, but not in the presence of large amounts of detergent. Common contaminants, such as reducing reagents (e.g., DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, and DNA (but not detergents), are well tolerated in the assay. Slight protocol modifications are required for other contaminants.

The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration.

Which product should you choose for fluorometric protein quantitation?
1–20 samples: use this Qubit Protein Assay Kit with the Qubit Fluorometer
20–2000 samples: use the Quant-iT Protein Assay Kit with a microplate reader

Qubit Protein BR Assay Kit
The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining the protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures using a Qubit 4 Fluorometer. This protein kit can deliver accurate protein quantitation across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods. The assay is compatible with many common contaminants, such as detergents, reducing reagents (e.g., DTT, β-mercaptoethanol), salts, and nucleic acids.

Key features of the Qubit Protein BR Assay Kit include:
Broad dynamic range— 0.1–20 mg/mL, measure samples without dilution
Two-point standard curve— eliminate time-consuming standard preparation
Automatic concentration determination— receive protein concentration instantaneously; no need for offline calculations
Compatible— accurate in the presence of reducing reagents, detergents, and other common buffer components

The Qubit Protein BR Assay Kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 µL of Protein BR Assay Buffer to 10 or 20 µL of your sample, followed by 30 µL of Protein BR Assay Reagent. Incubate for 10 min at room temperature. Then insert the sample into a Qubit 4 instrument and read the concentration.

Notes:
1. The Qubit Protein Assay Kit can be used with Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. The Qubit Protein BR Assay Kit is a fluorescence-based assay intended for use only with the Qubit 4 Fluorometer.
3. 500 µL thin-walled clear PCR tubes (Cat. No. Q32856) are required but not included in the Protein Assay and BR Assay Kits.
For Research Use Only. Not for use in diagnostic procedures.

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Frequently asked questions (FAQs)

Citations & References