Qubit™ 3 Fluorometer

Catalog number:  Q33216

Invitrogen™  Related applications: Nucleic Acid Quantitation | Protein Assays and Analysis

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The Qubit 3 Fluorometer is the next generation of the popular benchtop fluorometer that accurately measures DNA, RNA, and protein using the highly sensitive fluorescence-based Qubit quantitation assays. The fluorescent dyes used in these assays emit signals only when bound to specific target molecules, even at low concentrations, thus minimizing the effects of contaminants, including degraded DNA or RNA. The integrated design of the instrument and assays results in quantitation that is far more sensitive than UV absorbance, making this system essential for quantitation of precious samples (samples that are rare, difficult to purify, or expensive to either obtain or prepare) or samples for "delicate" applications (samples for downstream assays that are extremely sensitive to sample conditions).

Key features of the Qubit 3 Fluorometer include:
• Powerful dual-core processor quickly and accurately quantifies DNA, RNA, and protein in less than 5 seconds per sample
• Uses as little as 1 μL of sample
• Stores up to 1000 sample results
• Large, 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own with the MyQubit software and web tool
• Small footprint saves space on your bench
• Language of your choice including English, French, Spanish, German, simplified Chinese and Japanese
• Android-based operating system

The Qubit 3 Fluorometer can also be used to directly measure the fluorescence of samples. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 3 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM Sequencer.

Enables more accurate results than with UV absorbance at low concentrations
The Qubit 3 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Save samples—and bench space
The thoughtfully designed Qubit 3 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 3 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a USB thumb drive and cable for data transfer to Excel® software and for software downloads, a universal power supply, four plug adapters, and electromagnetic CE certification.

Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick, simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 3 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only 2 minutes for DNA and RNA assays. 500- µL thin-walled PCR tubes (Cat. No. Q32856) are required.

System specifications include:
Instrument dimensions: 5.4"(w) x 10"(l) X 2.2" (h) (13.6 cm X 25 cm X 5.5 cm)
Weight: 743 g
Dynamic range: 5 orders of magnitude
Processing time: ≤5 seconds/sample
Light sources: blue LED (max ~470 nm), red LED (max ~635 nm)
Excitation filters: blue 430-495 nm, red 600-645 nm
Emission filters: green 510-580 nm, red 665-720 nm
Detectors: photodiodes, measurement capability from 300-1,000 nm
Warm-up time: <35 seconds
USB thumb drive: 4 GB
For Research Use Only. Not for use in diagnostic procedures.


Capacity: 1 assay tube per read
Dimensions: 5.4" (w) x 10" (l) x 2.2" (h); (13.6 cm x 25 cm x 5.5 cm)
Display Interface: 5.7" Capacitive touch screen
For Use With (Equipment): Qubit® Fluorometer
Format: Tube(s)
Input Voltage: 100-240 VAC
Instrument Memory: 1000 Samples
Product Line: Qubit™
Product Size: 1 each
Shipping Condition: Room Temperature
Weight: 743.0 g


Manuals & protocols

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