Search
Search
View additional product information for Qubit™ 4 NGS Starter Kit - FAQs (Q33228)
20 product FAQs found
Absorbance readers cannot distinguish small fragments, degraded samples, or single nucleotides from intact nucleic acid. The dyes in the kits only detect intact strands longer than about a 20-mer. The kits are only recognizing intact DNA or RNA, and ignoring degraded sample.
Please download and install the most current version of the Qubit Firmware from our website: https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources.html
For Qubit Fluorometers 1.0, 2.0 and 3.0 go to this web link: https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources/previous-qubit-models.html
For the Qubit 1.0 instrument, if it is connected to a computer, disconnect it from the computer, unplug it, wait at least 10 seconds, then plug it back in.
For the Qubit 2.0, 3.0 and 4.0 instruments, power off or unplug the instrument if possible, wait at least 10 seconds, then plug it back in. Do not unplug the instrument or remove the USB stick during a firmware update.
You can try to use a larger amount of sample if possible (up to 20 µL). Alternatively, if you are using the Qubit DNA BR Assay or Qubit RNA BR Assay, try using the Qubit DNA HS Assay or Qubit RNA HS Assay, as these have higher sensitivity.
Dilute your sample, or use a smaller volume of sample in the assay and try again. Alternatively, if you are using the Qubit DNA HS Assay or the Qubit RNA HS Assay, try using the Qubit DNA BR Assay or Qubit RNA BR Assay, as these have lower sensitivity.
If this occurs, view the raw fluorescence values for the standards and samples under “Check Standards” or “Check Calibration”. Confirm that the values for the samples fall between the values of the standards (or a little above the highest standard). If they do not, the sample is out of the accurate range of the assay. The Qubit Fluorometer screen should indicate OUT OF RANGE.
There are several reasons why this message may appear. Please check the following on your instrument:
1.View the raw fluorescence value for the standards under “Check Standards” or “Check Calibration”. Make sure that the value of Standard 2 is at least 10- to 50-fold higher than the value of Standard 1. For the protein assay, make sure that the value of Standard 3 is about twice the value of Standard 2.
2.Check to see that you have used the tubes in the correct order.
3.Check to see that you used 10 µL of the standard and 190 µL of the Qubit dye working solution in the appropriate tubes.
4.For RNA assays, try an unopened tube of Standard 2 in case the open RNA standard tube has degraded.
5.Check the age of the Qubit assay kit and standards. Kits are stable for at least 6 months from the date of receipt. If the kit is older than a year and the Qubit Fluorometer will not read the standards, it may need to be replaced with a new kit.
6.Check to see if the instrument is working properly. Lift the lid. Run a new calibration for any assay. You should see a red light (for RNA assays) or a blue light (for all other assays) flash 5 times.
Yes, the manual has directions for this application.
Yes. We have seen a 20-30% difference. For the different forms of plasmid DNA, we recommend using a standard that more closely represents the composition of the plasmid DNA in the sample. For information on creating custom optimized assays, please visit our website (http://www.thermofisher.com/search/global/searchAction.action?query=MyQubit&resultPage=1&resultsPerPage=15&autocomplete).
Yes. Use the Qubit DNA BR Assay for a typical plasmid miniprep with a high concentration of DNA (over 50 ng/µL). Use the Qubit DNA HS Assay for plasmid rescue or methods that yield only small amounts of DNA. However, see the next question for conformation issues.
The USB that comes with the Qubit 2.0 Fluorometer is 2 GB, and the one that comes with the Qubit 3.0 and 4.0 Fluorometer is 4 GB.
We will replace the instrument. Please contact our Technical Support department for details by emailing techsupport@thermofisher.com.
No. The warranty will be voided if the instrument is disassembled or if you attempt to repair the instrument. It is best to call or email our technical support team for assistance.
There are two light sources in the Invitrogen Qubit Fluorometers - both are LEDs. They are expected to last at least 5 years.
The diluted standards can be used for up to 3 hours if using the same working solution for the samples. The Qubit reagents diluted in buffer are stable for up to 3 hours. Evaporation would result in a higher concentration of dye and sample, if present, and this would affect the results.
The Invitrogen Qubit 3.0 and 4.0 Fluorometers can store up to 1,000 samples' worth of data in a .csv file.
The Invitrogen Qubit 2.0 Fluorometer can store up to 200 lines of data in a .csv file.
The original Invitrogen Qubit (1.0) Fluorometer can store up to 20 lines of data in a .csv file.
Yes, these kits will work with all Qubit Fluorometers.
Yes, the Qubit 4.0 Fluorometer comes with both a USB and cable to connect to a computer.
Yes, the Invitrogen Qubit&trade 3.0 Fluorometer will be discontinued.
The Qubit Fluorometer quantifies DNA, RNA, and protein with superior accuracy, sensitivity, and simplicity. It is designed for molecular biology labs working with precious samples that are rare or difficult to process, and applications requiring precise measurement such as real-time PCR. See more about the Qubit Fluorometer (http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-fluorometer.html.html).