ViewRNA™ ISH Cell Assay Kit
ViewRNA™ ISH Cell Assay Kit
Citas y referencias (27)
Invitrogen™

ViewRNA™ ISH Cell Assay Kit

El ensayo celular ViewRNA™ ISH es un método de hibridación in situ de ARN de fluorescencia directa que permite laMás información
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Número de catálogoCantidad
QVC00011 kit
Número de catálogo QVC0001
Precio (MXN)
-
Cantidad:
1 kit
El ensayo celular ViewRNA™ ISH es un método de hibridación in situ de ARN de fluorescencia directa que permite la detección simultánea de uno a cuatro objetivos de ARN (ya sea ARNm o ARN no codificante) con sensibilidad de copia única y resolución de célula única mediante microscopios de fluorescencia o generadores de imágenes de alto contenido. A diferencia de las técnicas tradicionales FISH, que generalmente están limitadas por un fondo alto y una sensibilidad baja, el ensayo utiliza química patentada para los conjuntos de sondas específicas (sondas de ARN FISH) y para la amplificación de la señal de ADN ramificado (ADNb) para la detección de una señal específica. El ensayo de hibridación in situ fluorescente del ARN consta de cuatro pasos principales: preparación de la muestra, hibridación del objetivo, amplificación de la señal y detección. El kit de ensayo ViewRNA ISH puede detectar hasta tres ARN, pero se puede combinar con el módulo ViewRNA™ 740 para añadir una cuarta sonda.

Componentes adicionales necesarios:
10X PBS (n.º de cat QVC0508)
Solución detergente QC ViewRNA™ (n.º de cat. QVC0509)
Juego de tampones de lavado ViewRNA™ (n.º de cat. QG0507)
El kit de accesorios para células ViewRNA™ ISH (n.º de cat. QVC0700) está diseñado para proporcionar muchos de los componentes necesarios para realizar el ensayo, no suministrados en el kit de reactivos.
Los juegos de sondas ViewRNA™ no se incluyen en el ensayo y están diseñados para usarse con los ensayos de células ViewRNA ISH. Visite nuestro sitio web para ver una lista completa de más de 6500 juegos de sondas sintetizados. Con petición previa, se pueden diseñar y sintetizar nuevos juegos de sondas en menos de dos semanas sin costes adicionales.

Para añadir una cuarta sonda de ARN:
El módulo celular 740 ViewRNA™ ISH (n.º de cat. QVC0200) está diseñado para utilizarse junto con el ensayo celular ViewRNA™ ISH y permite el análisis de un objetivo de ARN adicional en el canal 740 (AlexaFluor 750) Consulte el prospecto para obtener una lista completa de los materiales suministrados en el kit.

Aplicaciones declaradas
Microscopía

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Cantidad1 kit
TipoKit de ensayo de células ISH
Unit SizeEach
Contenido y almacenamiento
Componentes suficientes para ensayos de 1-plex a 3-plex (AlexaFluor 488, 546 y 647) en placas de 24 pocillos (24 muestras), 96 ensayos cuando se utilizan cubreobjetos montados sobre portaobjetos de vidrio

Preguntas frecuentes

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The ViewRNA ISH Cell Assay Kit user manual lists an accessory kit, Cat. No. QVC0700 which has been discontinued. Do you offer or suggest alternatives for the items in the accessory kit that have also been discontinued?

Please contact our Technical Support team at cellanalysis.support@thermofisher.com for the list of suggested alternatives.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ViewRNA™ ISH Cell Assay Kit FAQs

Citations & References (27)

Citations & References
Abstract
Raphe serotonin neuron-specific oxytocin receptor knockout reduces aggression without affecting anxiety-like behavior in male mice only.
Authors:Pagani JH, Williams Avram SK, Cui Z, Song J, Mezey É, Senerth JM, Baumann MH, Young WS
Journal:
PubMed ID:25677455
'Serotonin and oxytocin influence aggressive and anxiety-like behaviors, though it is unclear how the two may interact. That the oxytocin receptor is expressed in the serotonergic raphe nuclei suggests a mechanism by which the two neurotransmitters may cooperatively influence behavior. We hypothesized that oxytocin acts on raphe neurons to influence ... More
Frequent proviral integration of the human betaretrovirus in biliary epithelium of patients with autoimmune and idiopathic liver disease.
Authors:Wang W, Indik S, Wasilenko ST, Faschinger A, Carpenter EJ, Tian Z, Zhang Y, Wong GK, Mason AL
Journal:
PubMed ID:25521721
'A human betaretrovirus (HBRV) has been linked with primary biliary cirrhosis (PBC) following the detection of viral particles in biliary epithelium by electron microscopy and cloning of the betaretrovirus genome from biliary epithelium and peri-hepatic lymph nodes. Evidence for viral infection was found in the majority of PBC patients'' peri-hepatic ... More
High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry.
Authors:Porichis F, Hart MG, Griesbeck M, Everett HL, Hassan M, Baxter AE, Lindqvist M, Miller SM, Soghoian DZ, Kavanagh DG, Reynolds S, Norris B, Mordecai SK, Nguyen Q, Lai C, Kaufmann DE
Journal:
PubMed ID:25472703
Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single-cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human leukocytes. This technique can ... More
RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.
Authors:Yu M, Ting DT, Stott SL, Wittner BS, Ozsolak F, Paul S, Ciciliano JC, Smas ME, Winokur D, Gilman AJ, Ulman MJ, Xega K, Contino G, Alagesan B, Brannigan BW, Milos PM, Ryan DP, Sequist LV, Bardeesy N, Ramaswamy S, Toner M, Maheswaran S, Haber DA
Journal:Nature
PubMed ID:22763454
Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. Although these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse ... More
Urinary Xist is a potential biomarker for membranous nephropathy.
Authors:Huang YS, Hsieh HY, Shih HM, Sytwu HK, Wu CC
Journal:
PubMed ID:25157805
Membranous nephropathy (MN), a type of glomerular nephritis, is the most common cause of nephrotic syndrome in human adults. Changes in gene expression as a result of epigenetic dysregulation through long noncoding RNAs (lncRNAs) are increasingly being recognized as important factors in disease. Using an experimental MN mouse model, we ... More
View all ViewRNA™ ISH Cell Assay Kit citations