ViewRNA™ ISH Cell Assay Kit

Zitierungen und Referenzen (27)

Invitrogen™

ViewRNA™ ISH Cell Assay Kit

Der ViewRNA™ ISH Zell-Assay ist eine direkte Fluoreszenz-RNA-In-situ-Hybridisierungsmethode, die den gleichzeitigen Nachweis von ein bis vier RNA-Zielen (entweder mRNA oderWeitere Informationen

Katalognummer	Menge
QVC0001	1 Kit

Katalognummer QVC0001

Preis (EUR)

1.518,65

Exklusiv online

1.726,00

Ersparnis 207,35 (12%)

Zum Warenkorb hinzufügen

Menge:

1 Kit

Preis (EUR)

1.518,65

Exklusiv online

1.726,00

Ersparnis 207,35 (12%)

Zum Warenkorb hinzufügen

Der ViewRNA™ ISH Zell-Assay ist eine direkte Fluoreszenz-RNA-In-situ-Hybridisierungsmethode, die den gleichzeitigen Nachweis von ein bis vier RNA-Zielen (entweder mRNA oder nicht-kodierende RNA) bei Single-Copy-Empfindlichkeit und Einzelzellauflösung mit Fluoreszenzmikroskopen oder High-Content-Imagern ermöglicht. Im Gegensatz zu herkömmlichen FISH-Verfahren, die im Allgemeinen durch einen hohen Hintergrund und eine geringe Empfindlichkeit begrenzt sind, verwendet der Assay proprietäre Chemie für die zielspezifischen Sondensets (RNA FISH-Sonden) und die Branch-DNA-Signalamplifikation (bDNA) zum Nachweis spezifischer Signale. Der Fluoreszenz-RNA-in-situ-Hybridisierungsassay umfasst vier Hauptschritte: Probenvorbereitung, Zielhybridisierung, Signalamplifikation und Signalerkennung. Dieses ViewRNA ISH Assay-Kit kann bis zu drei RNA erkennen, kann aber mit dem ViewRNA™ 740 Modul kombiniert werden, um eine vierte Sonde hinzuzufügen.

Erforderliche Zusatzkomponenten:
10x PBS (Kat.-Nr. QVC0508)
ViewRNA™ QK-Reinigungslösung (Kat. Nr. QVC0509)
ViewRNA™ Waschpuffer-Set (Kat.-Nr. QG0507)
ViewRNA™ ISH Zellzubehör-Kit (Kat.-Nr. QVC0700), in dem viele der erforderlichen Komponenten enthalten sind, die nicht im Reagenzienkit vorkommen, um den Assay durchzuführen.
ViewRNA™ Sondensets sind nicht im Assay enthalten und für die Verwendung mit ViewRNA ISH Zell-Assays konzipiert. Besuchen Sie unsere Website, um eine vollständige Liste von über 6.500 synthetisierten Sondensets zu erhalten. Auf Wunsch können neue Sondensätze in weniger als zwei Wochen ohne zusätzliche Kosten entwickelt und synthetisiert werden.

So fügen Sie die vierte RNA-Sonde hinzu:
Das ViewRNA™ ISH Cell 740 Modul (Kat.-Nr. QVC0200) wurde für die Verwendung in Verbindung mit dem ViewRNA™ ISH Zell-Assay entwickelt und ermöglicht die Analyse eines zusätzlichen RNA-Ziels im 740-Kanal (AlexaFluor 750). Eine vollständige Liste der im Kit enthaltenen Materialien finden Sie in der Packungsbeilage.

Berichtete Anwendungen
Mikroskopie

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Menge1 Kit

TypISH Zellassay-Kit

Unit SizeEach

Inhalt und Lagerung

Ausreichend Komponenten für 1-plex- bis 3-plex-Assays (AlexaFluor 488, 546 und 647) im 24-Well Plattenformat (24 Proben) und 96 Assays bei Verwendung von Deckgläsern auf Glasobjektträgern.

Häufig gestellte Fragen (FAQ)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The ViewRNA ISH Cell Assay Kit user manual lists an accessory kit, Cat. No. QVC0700 which has been discontinued. Do you offer or suggest alternatives for the items in the accessory kit that have also been discontinued?

Please contact our Technical Support team at cellanalysis.support@thermofisher.com for the list of suggested alternatives.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ViewRNA™ ISH Cell Assay Kit FAQs

Zitierungen und Referenzen (27)

Zitierungen und Referenzen

Abstract

Raphe serotonin neuron-specific oxytocin receptor knockout reduces aggression without affecting anxiety-like behavior in male mice only.

Authors:Pagani JH, Williams Avram SK, Cui Z, Song J, Mezey É, Senerth JM, Baumann MH, Young WS

Journal:

PubMed ID:25677455

'Serotonin and oxytocin influence aggressive and anxiety-like behaviors, though it is unclear how the two may interact. That the oxytocin receptor is expressed in the serotonergic raphe nuclei suggests a mechanism by which the two neurotransmitters may cooperatively influence behavior. We hypothesized that oxytocin acts on raphe neurons to influence ... More

Frequent proviral integration of the human betaretrovirus in biliary epithelium of patients with autoimmune and idiopathic liver disease.

Authors:Wang W, Indik S, Wasilenko ST, Faschinger A, Carpenter EJ, Tian Z, Zhang Y, Wong GK, Mason AL

Journal:

PubMed ID:25521721

'A human betaretrovirus (HBRV) has been linked with primary biliary cirrhosis (PBC) following the detection of viral particles in biliary epithelium by electron microscopy and cloning of the betaretrovirus genome from biliary epithelium and peri-hepatic lymph nodes. Evidence for viral infection was found in the majority of PBC patients'' peri-hepatic ... More

High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry.

Authors:Porichis F, Hart MG, Griesbeck M, Everett HL, Hassan M, Baxter AE, Lindqvist M, Miller SM, Soghoian DZ, Kavanagh DG, Reynolds S, Norris B, Mordecai SK, Nguyen Q, Lai C, Kaufmann DE

Journal:

PubMed ID:25472703

Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single-cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human leukocytes. This technique can ... More

RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.

Authors:Yu M, Ting DT, Stott SL, Wittner BS, Ozsolak F, Paul S, Ciciliano JC, Smas ME, Winokur D, Gilman AJ, Ulman MJ, Xega K, Contino G, Alagesan B, Brannigan BW, Milos PM, Ryan DP, Sequist LV, Bardeesy N, Ramaswamy S, Toner M, Maheswaran S, Haber DA

Journal:Nature

PubMed ID:22763454

Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. Although these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse ... More

Urinary Xist is a potential biomarker for membranous nephropathy.

Authors:Huang YS, Hsieh HY, Shih HM, Sytwu HK, Wu CC

Journal:

PubMed ID:25157805

Membranous nephropathy (MN), a type of glomerular nephritis, is the most common cause of nephrotic syndrome in human adults. Changes in gene expression as a result of epigenetic dysregulation through long noncoding RNAs (lncRNAs) are increasingly being recognized as important factors in disease. Using an experimental MN mouse model, we ... More

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