Thermo Scientific™

Aminoallyl-dUTP Solution (10 mM)

Catalog number:  R0091

Related applications:

Nucleic Acid Labeling & Oligo Synthesis

Back to top

Description

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Applications

• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration (molarity): 10 mM
Label or Dye: Aminoallyl
Labeling Method: Direct Labeling
Product Size: 0.1 mL