Thermo Scientific™

Anaerobic KV Blood Agar (CDC formulation) with Kanamycin and Vancomycin

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Clinical Microbiology

Inhibit gram-positive microorganisms and facultative anaerobic bacteria and select for gram-negative bacilli with Thermo Scientific™ Remel™ Anaerobic KV Blood Agar [The Centers for Disease Control and Prevention (CDC) formulation] with kanamycin and vancomycin. Formulated by V.R. Dowell and T.M Hawkins at the CDC in Atlanta Georgia1, Anaerobic Blood Agar (CDC) supports the growth and typical pigmentation of fastidious, slow-growing and other clinically important anaerobes.

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Isolate and cultivate anaerobic organisms, including fastidious strains using the solid medium Remel Anaerobic KV Blood Agar (CDC formulation) with kanamycin and vancomycin.

  • Selective: Kanamycin and vancomycin are antibiotics, which inhibit gram-positive organisms and facultative anaerobic bacteria and select for gram-negative bacilli.
  • Ready-to-use: Convenience of prepared media.

Anaerobic Blood Agar (CDC) base contains peptones that supply nitrogenous substances and amino acids necessary for the growth of anaerobic bacteria. Yeast extract provides B-complex vitamins and serves as a growth enhancer. Hemin, vitamin K and sheep blood stimulate the growth of anaerobes. Sodium chloride is a source of essential electrolytes and maintains osmotic equilibrium. Kanamycin and vancomycin are antibiotics, which inhibit gram-positive organisms and facultative anaerobic bacteria and select for gram-negative bacilli. Laked blood is used in anaerobic media to enhance the pigmentation of anaerobic organisms2. Neomycin aids in the isolation of anaerobic organisms, such as Bacteriodes and Clostridium species, and inhibits gram-negative Enterobacteriaceae. Phenylethly alcohol (PEA) reduces the growth of facultative gram-negative anaerobes while allowing the growth of obligate anaerobic bacteria3. Paromomycin and vancomycin facilitate the recovery of fastidious, obligately anaerobic non-spore-forming gram-negative bacilli from mixed populations by inhibiting facultative and aerobic gram-negative rods4.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

1. Dowell, V.R. and T.M Hawkins. 1974. Laboratory Methods in Anaerobic Bacteriology, CDC, Laboratory Manual, US Dept. of H.H.S and CDC, Atlanta, GA .
2. Finegold S.M., Miller, A.B. and Posnick, D.J. 1965. Ehrährungsforschung. 10:517–528.
3. Dowell, V.R., Jr.., Hill, C.O. and Altemeier, W.A. 1964. J Bacteriol. 88:1811–1813.
4. Dowell, V.R., Jr., Lombard, G.L., Thompson, F.S. and Armfield, A.Y. 1981. Media for Isolation, Characterization and Identification of Obligately Anaerobic Bacteria. US Dept. of H.H.S and CDC, Atlanta, GA.