Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity—confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR
• Applications
• For use in all molecular biology applications, including PCR, real-time PCR and RT-PCR with Taq DNA polymerase, cDNA synthesis, and primer extension.

Note: dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.