DNase Test Agar w/Methyl Green

Detect the deoxyribonuclease (DNase) activity in microorganisms with Thermo Scientific™ Remel™ DNase Test Agar w/Methyl Green.

In 1957, Jeffries et al. developed a medium to demonstrate DNase activity of microorganism that possess this extracellular enzyme¹. This medium is used to identify a variety of bacteria including staphylococci, enteric gram-negative bacilli and pseudomonads^2-5. On DNase Test Agar w/Methyl Green, the colony is surrounded by a clear zone in an otherwise green medium⁶.

DNase Test Agar w/Methyl Green is a solid medium used to detect the deoxyribonuclease (DNase) activity in microorganisms. For the methyl green modification, the dye methyl green is able to bind to polymerized DNA to form a stable green complex at pH 7.3. The action of DNase depolymerizes the DNA structure releasing methyl green; when not combined at this pH methyl green becomes colorless resulting in colorless halos around DNase-positive colonies.

• Easy-to-interpret:
  • Positive test – Growth line surrounded by a colorless zone in an otherwise green agar plate.
  • Negative test – Growth line surrounded by no zone, the entire plate is green.
• Ready-to-use: convenience of prepared media.

DNase Test Agar w/Methyl Green contain casein and soy peptone that supply nitrogen, amino acids and peptides necessary for bacterial growth. Sodium chloride provide essential electrolyte which maintain osmotic equilibrium.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.