Isolate and detect Listeria monocytogenes from clinical, food and environmental samples with the diagnostic Thermo Scientific™ Remel™ Modified Oxford Agar (MOX). In 1989, Curtis described Oxford Agar, a selective medium for Listeria monocytogenes1. Lee and McClain modified Oxford Agar by replacing the original selective agents with moxakactam2. This medium is recommended by the U.S. Department of Agriculture Food Safety Inspection Service and American Public Health Association (APHA) for use following enrichment procedures for the recovery of L. monocytogenes from meat and dairy products3, 4.
Foodborne infection by Listeria monocytogenes has prompted increased concern for detecting this organism in foods, in the environment and in pathological specimens from both human and animal subjects. The ability to isolate the organism has been impeded in the past by lack of an effective selective medium, as Listeria monocytogenes can be easily and completely overgrown by competing flora.
Use Modified Oxford Agar (MOX) for selective isolation and detection of Listeria monocytogenes from clinical and food specimens.
L. monocytogenes hydrolyze esculin to form6, 7, dihydroxyumarin which reacts with ferric ions to form a black precipitate in the medium surrounding the colonies. Lithium chloride, colistin, and moxalactam are selective agents which inhibit the growth of background organisms, such as Gram-positive cocci and gram-negative bacilli.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
1. Curtils, G.D.W., R.G. Mitchell, A.F. King, and E.J. Griffin. 1989. Appl. Microbiol. 8:95-98.
2. Lee, W.H. and D. McClain. 1986. AAPL. Environ. Microbiol. 52: 1215 -1217
3. Downes, F.P. and K. Ito. Compodium of Methods for the Microbiological Examination of Foods. 4th ed. APHA, Washington, D.C.
4. U.S. Department of Agriculture Food Safety and Inspection Service. 1998. Microbiology Laboratory Guidebook. 3rd. ed. Chap 8, (revised 8/05).