Isolate and cultivate pathogenic yeast and molds from clinical specimens with Thermo Scientific™ Remel™ Sabouraud Dextrose Agar (Emmons) prepared media plates. Sabouraud Dextrose Agar (Emmons) is a modification of the original medium developed by Sabouraud in 18921. Emmons neutralized the pH, increasing it to 7.0, and reduced the concertation of dextrose from 40 to 20 g/L2.
Use Sabouraud Dextrose Agar (Emmons) for primary isolation and cultivation of pathogenic yeasts and molds from clinical specimens.
Casein and meat peptones supply nitrogenous compounds and amino acids necessary for the growth of fungi. Dextrose is an energy source which serves to support the growth of fungi. Chloramphenicol and gentamicin are broad-spectrum antibiotics which inhibit a range of Gram-positive and Gram-negative bacteria. Cycloheximide is inhibitory to rapidly growing saprophytic fungi that could overgrow slow-growing primary pathogens. Penicillin is active against Gram-positive bacteria. Streptomycin inhibits Gram-positive and Gram-negative bacteria.
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1. Sabouraud,R. 1892. Ann.Dermatol.Syphil. 3:1061 – 1987
2. Emmons, C.W., S.H.Binford, J.P.Utz, and K.J. Kwon-Chung. 1977. Medical Microbiology. 3rd ed. Lea and Febiger, Philadelphia, PA.