Extended Spectrum β Lactamases are enzymes capable of conferring bacterial resistance to penicillins, cephalosporins, and aztreonam by hydrolysis of these antibiotics 1,2
. Production of ESBLs is a significant resistance-mechanism that impedes the antimicrobial treatment of infections caused by Enterobacteriaceae
and is a serious threat to the currently available antibiotic armory 3
Spectra ESBL Medium supports HAI surveillance programs through fast isolation and presumptive identification of ESBL-producing bacteria in clinical samples.
- Easy-to-read: clearly differentiates ESBL-producing organisms by their blue/turquoise-green or pink colonies.
- Facilitates early intervention: fast, accurate results support infection prevention and control.
- High specificity: reduces costs associated with unnecessary isolation and additional testing.
- Streamlined workflow: screen positive within 18 to 24 hours directly from perirectal swabs or fresh stool specimens.
- Ready-to-use: convenience of prepared media.
- First FDA-cleared chromogenic media for the presumptive identification of Proteus.
A mixture of chromogens forms a substrate for two enzymes: β-galactosidase and glucuronidase that are differentially expressed in different species of bacteria resulting in blue/turquoise-green or pink colonies. Other ESBL-producing organisms that do not utilize the chromogenic substrates may produce tan colonies through deamination of tryptophan. Non-target organisms generally appear cream colored or are naturally pigmented green or brown.
The main ESBL-producing bacteria include: Escherichia coli
, Klebsiella pneumoniae
, Klebsiella oxytoca
and Proteus mirabilis
. Accurate and rapid detection of ESBL-producing bacteria is essential to assist in the selection of appropriate antibiotic therapy, avoid treatment failures in patients, and prevent further transmission of these multi-drug-resistant organisms.4,5
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1. Paterson, D.L. and R.A. Bonomo. 2005. Clin. Microbiol. Rev. 18:657-686.
2. Clinical and Laboratory Standards Institute (CLSI). 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 9th ed. M07-A9. CLSI, Wayne, PA.
3. Shaiks,S., Jamale,F., Shakil, S., Rizvi, S.M.D., Kama, M.A 2014 Saudi J Biol Sci. 2015 Jan; 22(1): 90–101. 4. Harris, A.D., J.C. McGregor, J.A. Johnson, S.M. Strauss, A.C. Moore, H.C. Standiford, J.N. Hebden, and J.G. Morris. 2007. Emerg. Infect. Dis. 57:310-315.
5. Thomson. K. S. 2010. Extended-Spectrum-β-Lactamases, AmpC, and Carbapenamase Issues.J.Clin.Microbiol,48:1019-1025