Thermo Scientific™

Nocardia Quad

Catalog number: R02462
Thermo Scientific™

Nocardia Quad

Catalog number: R02462

Differentiate aerobic actinomycetes by hydrolysis of casein, xanthine, tyrosine, and starch with Thermo Scientific™ Remel™ Nocardia Quad. A taxonomic scheme to differentiate these genera based on biochemical characteristics was developed by Mishra et al1. McGinnis et al. simplified it to identify the most commonly isolated actinomycetes by including tests for casein, xanthine, and tyrosine degradation2. Starch is added to Nutrient Agar to presumptively differentiate Streptomyces from Nocardia based on the inability of Nocardia species to hydrolyze starch3.

 
Catalog Number
R02462
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Each
dimFormat
Price (USD)
Full specifications
DescriptionNocardia Quad
FormatQuadplate
Product TypeAgar
Quantity10/Pk.
Unit SizeEach
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Catalog NumberSpecificationsUnit SizedimFormatPrice (USD)
R02462Full specifications
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DescriptionNocardia Quad
FormatQuadplate
Product TypeAgar
Quantity10/Pk.
Unit SizeEach
Showing 1 of 1

The aerobic actinomycetes as a group are gram-positive, catalase-positive bacteria that may be presumptively identified by staining properties, microscopic morphology, and substrate degradation. Certain actinomycetes produce a hydrolytic enzyme that degrades the skim milk in Casein Agar, which is demonstrated by clearing of the agar surrounding the area of growth.

  • Ready to use – Solid media
  • Convenient – Quad plate format
  • Space saving – Read more results from a single sample and produce more diagnostic details while reducing storage capacity issues in the incubator with a quad-plate that includes four separate media formulations
  • Easy to interpret
    • Casein Agar (I)
      • Positive Test – Clearing of casein agar around and/or under growth
      • Negative Test – Growth with no clearing of the medium
    • Xanthine Agar (II)
      • Positive Test – Clearing of xanthine agar around and/or under growth
      • Negative Test – Growth with no clearing of the medium
    • Tyrosine Agar (III)
      • Positive Test – Clearing of tyrosine agar around and/or under growth
      • Negative Test – Growth with no clearing of the medium
    • Starch Agar (IV)
      • Positive Test – No color development surrounding growth
      • Negative Test - Purple color development surrounding growth

Casein agar contains skim milk that acts as a substrate for the hydrolytic enzyme produced by some actinomycetes.

Xanthine and Tyrosine Agars contain beef extract that provides carbohydrates, vitamins, nitrogen compounds, and salts. The peptones present in these media provide nutrients in the form of amino acids and peptides.

Starch Agar contains potato starch which acts as a substrate to detect amylase production. This is demonstrated by clearing of the agar surrounding the area of growth, and easily detected upon addition of Gram Iodine or Lugol’s iodine.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

  1. Mishra, S.K., R.E. Gordon, and D.A. Barrnett. 1980. J.Clin.Microbiol. 11:728.
  2. McGinnis, M.R., R.F. D’Amato, and G.A. Land. 1982. Pictorial Handbook of Medically Important Fungi and Aerobic Actinomycetes. Praeger Publishers, New York, NY.
  3. Versalovic, J., K.C. Carroll, G. Funke, J.H. Jorgensen, M. L. Landry, and D.W. Warnock. 2011. Manual of Clinical Microbiology. 10th ASM Press, Washington, D.C.

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