Presumptively identify anaerobic, gram-positive rods using Thermo Scientific™ Remel™ Anaerobic Gram Positive ID Quad (Lombard-Dowell Base)1. The Remel Anaerobic Gram Gram Positive ID Quad is based on the CDC’s Presumpto Plates2: I, II, and III, with three quadrants containing various differential media, which can be used to identify Clostridium spp3.
|Anaerobic Gram Positive ID Quad;
|Anaerobic Gram Negative ID Quad
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Presumptively identify anaerobic, gram-positive rods using Anaerobic Gram Positive ID Quad plates.
Quadrant I contains glucose, which detects fermentation and growth stimulation. Bromothymol blue monitors the fermentation reaction.
Quadrant II contains DNA, which is hydrolyzed by some obligately anaerobic bacteria. The colour change of toluidine blue indicates DNA hydrolysis.
Quadrant III contains starch to analyse starch hydrolysis by anaerobic bacteria.
Quadrant IV contains milk proteins (casein), which may be hydrolysed by anaerobic bacteria.
Lombard-Dowell Agar Base supplemented with the growth factors, vitamin K and hemin, supports the growth of common anaerobic bacteria. The cystine and sodium sulfite reduce the redox potential of the medium. Quadrant l contains glucose for the detection of fermentation and growth stimulation. Bromothymol blue serves as the indicator of acid production during fermentation by a color change to yellow. Quadrant II contains DNA which is hydrolyzed by some obligately anaerobic bacteria. Toluidine blue is an indicator which results in a pink zone around growth when DNA is hydrolyzed. Quadrant III contains starch to test the ability of anaerobic bacteria to hydrolyze starch, resulting in a clearing of the medium when it is flooded with iodine. Quadrant IV contains milk proteins (casein) which may be hydrolyzed by anaerobic bacteria, resulting in a clearing of the medium.
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