PRAS Chopped Meat Broth

Cultivate anaerobic microorganisms using Thermo Scientific™ Remel™ PRAS Chopped Meat Broth. R.E. Hungate developed the basis for these media, referred to as the roll tube technique. The use of a steam sterilized emulsion of a brain tissue in water to support the growth of anaerobic bacilli was first developed by von Hibler¹. Henry used the chopped meat broth for cultivating anaerobes and to study their saccharolytic and proteolytic properties². Recommended by the Food and Drug Administration (FDA) and by the Association of Official Analytical Chemists (AOAC)³.

Cultivate anaerobic microorganisms using Pre-reduced Anaerobically Sterilized (PRAS) Chopped Meat Broth. PRAS media are processed in a reduced condition and remain reduced up to and after inoculation⁴.

• Ready-to-use – Convenience of a prepared medium.
• Recommended by FDA for use in the enumeration and identification of Clostridium perfringens from food and by the AOAC for the detection of Clostridium botulinum³.
• Capacity to initiate growth of bacteria from minute inocula and maintain viability of cultures over extended periods of time.

This medium contains minimal salts, beef heart, peptone, and dextrose which provide reducing substances, amino acids and other nutrients necessary for the growth of anaerobes. Solid meat particles create favorable growth conditions for anaerobes due to the reducing action of sulfhydryl groups from muscle protein. Cysteine provides sulfahydryl groups which act as reducing agents to bind hydrogen ions and produce anaerobic conditions. Vitamin K and hemin are essential for the growth of certain anaerobes, specifically Prevotella melaninogenica. A mixed carbohydrate-starch source was developed to provide a carbon source for all anaerobes including those which do not use glucose. When glucose or other carbohydrates are incorporated, a growth medium results which is useful in obtaining rapid growth from single colonies for chromatographic analyses, susceptibility testing, or inoculation of different media.

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