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Differentiate and Identify Enterobacteriaceae spp. using Thermo Scientific™ Remel™ Gelatin Medium (Nutrient Gelatin). In their studies of gelatin liquefaction by gram-negative bacilli, Levine and Carpenter incorporated gelatin into the nutrient medium1. The production of gelatinase by some bacteria and the resulting liquefaction of gelatin can be used to differentiate certain bacilli. Gelatin Medium (Nutrient Media) (12%) is recommended as a standard method for use in taxonomic work because the rate of gelatin liquefaction is important in the characterization of the Enterobacteriaceae2.
| Description | Gelatin Medium |
| Format | 15mm x 103mm Tube |
| Product Type | Gelatin |
| Quantity | 20 x 5 mL |
| Unit Size | Each |
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Certain genera of enteric gram-negative bacilli and nonfermentive gram-negative bacilli can be differentiated and identified by the production of gelatinase. Gelatinase is a proteolytic enzyme that hydrolyzes gelatin into its constituent amino acids with a loss of its gelling characteristics3.
- Ready to use – Convenience of prepared media.
- Recommended as a standard method for use in taxonomic work because the rate of gelatin liquefaction is important in the characterization of the Enterobacteriaceae2.
- Easy to interpret -
- Positive test - Liquefacation after refrigeration
- Negative test - Solidification after refrigeration
The medium contains peptone and beef extract that supply amino acids and other essential nutrients to support the growth of non-fastidious bacteria. Gelatin is incorporated to determine an organism’s ability to produce gelatinase. The proteolytic enzyme gelatinase decomposes gelatin into smaller components, resulting in liquefaction of the gelatin.
- Levine, M. and D.C. Carpenter. 1923. J. Bacteriol. 8:297-306.
- Ewing, W.H. 1986. Edwards and Ewing’s Identification of Enterobacteriaceae. 4th Elsevier Science Publishing Co. Inc., New York, NY.
- MacFaddin, J.F. 2000. Biochemical Tests for Identification of Medical Bacteria. 3rd Lippincott Williams & Wilkins, Philadelphia, PA.