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Differentiate aerobic actinomycetes like Nocardia and Streptomyces using Thermo Scientific™ Remel™ Lysozyme Broth. A taxonomic scheme for identification of aerobic actinomycetes is developed based on biochemical characteristics which included testing for the growth in the presence of lysozyme1. The enzyme lysozyme breaks down the cell walls of certain aerobic actinomycetes such as Streptomyces spp. and are referred sensitive and will not grow in lysozyme broth. Lysozyme resistance is more reliable to differentiate Nocardia and Streptomyces2.
| Description | Lysozyme Broth |
| Format | 15mm x 103mm Tube |
| Product Type | Microbiology Media |
| Quantity | 20 x 5 mL |
| Unit Size | Each |
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The aerobic actinomycetes are a heterogenous group of genera and include species of Nocardia and Streptomyces3. As a group, the organisms are gram-positive, catalase-positive and may be presumptively identified by staining properties, microscopic morphology, and substrate degradation4. Lysozyme Broth contains lysozyme that inhibits the growth of some aerobic actinomycetes, but not all. Most Nocardia spp. are resistant and grow in the presence of lysozyme.
This medium contains beef extract, peptone, and glycerol that supply carbohydrates, vitamins, nitrogen compounds, salts, and amino acids necessary for the growth of aerobic actinomycetes.
- Mishra, S.K., R.E. Gordon, and D.A. Barnett. 1980. J. Clin. Microbiol. 11:728.
- McGinnis, M.R., R.F. D’Amato, and G.A. Land. 1982. Pictorial. Handbook of Medically Important Fungi and Aerobic Actinomycetes. Praeger Publishers, New York, NY.
- Isenberg, H.D. 2004. Clinical Microbiology Procedures Handbook. 2nd, Vol. 2. ASM Press, Washington, D.C.
- Forbes, B.A., D.F. Sahm, and A.S. Weissfeld. 2007. Bailey and Scott’s Diagnostic Microbiology. 12th Mosby Elseview, St. Louis, MO.