Determine oxidative and fermentative metabolism of sucrose by gram-negative bacilli using Thermo Scientific™ Remel™ OF Medium w/1% Sucrose (Hugh-Liefson). Hugh and Leifson first reported that microorganisms which utilize carbohydrates oxidatively produce only a minimal amount of acid1. Oxidative-Fermentative (OF) Medium was developed with a ratio of peptone to carbohydrate that minimized the formation of alkaline by-products and enhanced acid production by oxidative organisms. OF Medium is especially suitable for determining the carbohydrate metabolic pathway of gram-negative bacilli2.
|Certifications/Compliance||Industrial Reference: BAM.|
|Description||OF Medium w/1% Sucrose (Hugh-Liefson)|
|Format||15mm x 103mm Tube|
|Product Type||Microbiology Media|
|Quantity||20 x 4 mL|
|Catalog Number||Specifications||Unit Size||Quantity||Price (USD)|
|R061990||Each||20 x 4 mL||Request A Quote|
Typical fermentation media contain approximately 2% peptone and 1% carbohydrate. In media containing high concentrations of peptones, oxidative microorganisms produce alkaline by-products in quantities that neutralize the acid produced from carbohydrate metabolism.
OF Medium is a semisolid medium with a low peptone to carbohydrate ratio that contains the pH indicator bromthymol blue. Organisms which metabolize carbohydrate in OF medium produce acid in quantities sufficient to prevent its neutralization by alkaline peptone by-products.
OF Medium with Sucrose uses 4 mL of 1% sucrose as a fermentable carbohydrate. The medium contains casein peptone as the substrate and bromthymol blue as the pH indicator. Dipotassium phosphate promotes fermentation and also serves as a buffer. Agar allows for detection of motility and aids in even distribution of acid produced at the surface of the medium.
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