Determine fermentation reactions and metabolic end-products of anaerobic bacteria using the liquid medium Peptone Yeast Glucose Broth (PYG) with hemin and vitamin K.

Anaerobic infections can involve any organ in the body when appropriate condition exist³. Treatment of anaerobic infections is frequently different than aerobic bacteria, hence it is important to accurately identify the pathogenic bacteria⁴.

• This medium is processed in an atmosphere of nitrogen and hydrogen.
• Incubate under anaerobic conditions at 35-37°C for 48 hours, or until adequate growth is obtained.
• Gas liquid chromatography (GLC) is carried out for metabolic end product analysis and/or whole-cell fatty acid profilling.

Peptone Yeast Glucose Broth (PYG) with hemin and vitamin K contains peptone and yeast extract that supply protein and other nutrients required for the growth of anaerobic bacteria. Sodium bicarbonate and dipotassium phosphate are buffers. Glucose is an energy source. Vitamin K is required for growth of some anaerobic bacteria, like Bacteroides species and gram-positive sporeformers. Hemin supplies the X factor which stimulates the growth of many organisms. Cysteine helps to reduce and maintain low oxygen potential.

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