Regan Lowe Semi-Solid Transport

Selectively isolate Bordetella pertussis and Bordetella parapertussis with Thermo Scientific™ Remel™ Regan-Lowe Semi-Solid Transport Medium. Regan and Lowe demonstrated that charcoal agar supplemented with 40μg/mL cephalexin and 10% defibrinated horse blood was an excellent enrichment and transport medium¹. Hoppe reported that horse blood was superior to sheep or human blood for recovery of Bordetella spp. and incubation in ambient air results in more luxuriant growth than incubation in 5-10% CO₂².

B. pertussis and B. parapertussis that cause whooping cough, are amongst the most fastidious bacteria known and require special culturing techniques. Regan-Lowe transport medium is semi-solid in nature and is used for transportation and selective isolation of Bordetella pertussis and Bordetella parapertussis. The medium contains Cephalaxin which inhibits penicillin-resistant staphylococci, and some coliforms, as well as strains of Haemophilus influenzae and allows selective isolation of Bordetella spp. from respiratory specimens.

• Ready-to-use – Prepared media in 15mm x 103mm tubes (5mL)
• Selective – Cephalexin and Amphotericin B antibiotics inhibit penicillin resistant staphylococci, coliforms, Haemophilus influenzae; yeasts and fungi respectively and provides selective isolation of Bordetella spp.

Regan-Lowe Semi-Solid Transport Medium contains charcoal agar base with 10% lysed horse blood which provides an enriched medium. Nicotinic acid is added as an essential nutrient for Bordetella. Horse blood acts as a growth factor for B. pertussis and as a neutralizer of inhibiting substances². Cephalexin inhibits penicillin resistant staphylococci, coliforms and, Haemophilus influenzae. Amphotericin B inhibits growth of yeasts and fungi.

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.