Dermatophyte Test Medium (DTM)
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Thermo Scientific™
Dermatophyte Test Medium (DTM)

Facilitate selective and differential isolation of pathogenic fungi (dermatophytes) from cutaneous sources using Dermatophyte Test Medium (DTM).

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Catalog NumberFormatQuantity
R08352F - slant20/Pk.
R01365Monoplate Deep Pour10/Pk.
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Catalog number R08352
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Format:
F - slant
Quantity:
20/Pk.
Facilitate selective and differential isolation of pathogenic fungi (dermatophytes) from cutaneous sources using Thermo Scientific™ Remel™ Dermatophyte Test Medium (DTM). This medium was formulated by Taplin et al. for use in locations where specialized training and microscopic examination is not available1. A pH indicator and three antimicrobial agents are added to the agar to provide a differential and selective medium for isolation of dermatophytes belonging to the genera Microsporum, Trichophyton, or Epidermophyton.

Dermatophytes are fungi that possess keratinolytic properties that enable them to invade skin, nails and hair2. The infections caused by these organisms are commonly referred to as ringworm and are classified by the Latin word tinea followed by the area of the body infected3. The dermatophyte test medium (DTM) is recommended for selective isolation of pathogenic fungi from cutaneous sources.

  • Ready to Use: Convenience of prepared agar media.
  • Selective: Cyclohexamide, chloramphenicol, and gentamycin inhibit most saprophytic fungi and many gram-positive and gram-negative bacteria, including some Pseudomonas
  • Ease of identification: Color change of phenol red indicator from yellow to red

DTM is primarily used as a screening test for detection of dermatophytes. This medium contains soy peptone that supplies the nitrogen and carbon necessary for the growth of microorganisms. Dextrose acts as an energy source. Phenol red is a pH indicator which detects alkaline metabolites produced by dermatophytes, resulting in a color change from yellow to red. The three antibiotics cyclohexamide, chloramphenicol, and gentamycin are selective agents that inhibit growth of certain microorganisms.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

General References:

  1. Taplin, D., N. Zaias, G. Rebell, and H. Blank. 1969. Arch. Dermatol. 99:203-209.
  2. Murray, P.R., E.J. Baron, J.H. Jorgensen, M.L. Landry, and M.A. Pfaller. 2007. Maunal of Clinical Microbiology. 9th ed. ASM Press, Washington, D.C.
  3. Forbes, B.A., D.F. Sahm, A.S. Weissfled. Bailey and Scott's Diagnostic Microbiology. 12th ed. Mosby Elsevier, St. Louis, MO.
Specifications
DescriptionDermatophyte Test Medium
FormatF - slant
Product TypeMicrobiology Media
Quantity20/Pk.
Unit SizeEach