Thermo Scientific™


Catalog number:  R0851

 Related applications: PCR Cloning | Restriction Enzyme Cloning

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Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo.


• Detection of GUS expression in plant cells and tissues
• Detection of infections caused by E. coli
• Detection of bacterial contamination in food and water samples.

• Preparation of a 0.1 M stock solution in dimethylformamide or dimethylsulfoxide is recommended.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Colorimetric
Product Size: 0.1 g
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Glucuronidase (GUS) Substrate


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