DTT (dithiothreitol)
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DTT (dithiothreitol)
Thermo Scientific™

DTT (dithiothreitol)

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. ItRead more
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Catalog NumberQuantity
R08615 g
R086225 g
Catalog number R0861
Price (USD)/ Each
103.65
Online exclusive
121.00 
Save 17.35 (14%)
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Quantity:
5 g
Request bulk or custom format
Price (USD)/ Each
103.65
Online exclusive
121.00 
Save 17.35 (14%)
Add to cart
DTT (dithiothreitol)
Catalog numberR0861
Price (USD)/ Each
103.65
Online exclusive
121.00 
Save 17.35 (14%)
-
Add to cart
Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (see Reference 1).

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

Highlights

• Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases.

Applications

• Enzyme stabilization
• Enzymatic reactions
• Protein electrophoresis

Note
• DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.1 M
Product TypeDTT (Dithiothreitol)
Quantity5 g
Unit SizeEach