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Thermo Scientific™

DTT (dithiothreitol)

Catalog number: R0862
Thermo Scientific™

DTT (dithiothreitol)

Catalog number: R0862
Catalog Number
R0862
Unit Size
25 g
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
5 g
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R086225 g
-
R08615 g
-
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Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (see Reference 1).

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

Highlights

• Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases.

Applications

• Enzyme stabilization
• Enzymatic reactions
• Protein electrophoresis

Note
• DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
0.1 M
Product Type
DTT (Dithiothreitol)
Quantity
25 g

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