Quant-it™ RiboGreen Reagent and RNA Assay Kit
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Invitrogen™

Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberProduct TypeQuantitation Range
R11491Quant-iT RiboGreen RNA Reagent1 to 200 ng
R11490Quant-iT RiboGreen RNA Kit1 to 200 ng
R32700RediPlate 96 RiboGreen RNA Kit3 to 200 ng
Catalog number R11491
Price (USD)/ Each
662.65
Online exclusive
702.00 
Save 39.35 (6%)
-
Add to cart
Product Type:
Quant-iT RiboGreen RNA Reagent
Quantitation Range:
1 to 200 ng
Price (USD)/ Each
662.65
Online exclusive
702.00 
Save 39.35 (6%)
Add to cart
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Catalog numberR11491
Price (USD)/ Each
662.65
Online exclusive
702.00 
Save 39.35 (6%)
-
Add to cart
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200-2,000 cuvette assays using a 2 mL assay volume
Quantitation Range1 to 200 ng
Product LineQuant-iT, RIBOGREEN
Product TypeQuant-iT RiboGreen RNA Reagent
Quantity1 mL
Shipping ConditionRoom Temperature
Unit SizeEach