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Citas y referencias (14)
Invitrogen™
Resorufin β-D-Galactopyranoside
El sustrato fluorogénico β-galactosidasa resorufina β-D-galactopiranósido produce el producto de hidrólisis, la resorufina, con un máximo de absorción/emisión de 571/585Más información
| Número de catálogo | Cantidad |
|---|---|
| R1159 | 25 mg |
Número de catálogo R1159
Precio (MXN)
-
Cantidad:
25 mg
El sustrato fluorogénico β-galactosidasa resorufina β-D-galactopiranósido produce el producto de hidrólisis, la resorufina, con un máximo de absorción/emisión de 571/585 nm.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Excitación/emisión571⁄585
Cantidad25 mg
Condiciones de envíoTemperatura ambiente
SustratoSustrato Beta-Gal
Método de detecciónFluorescente
Substrate PropertiesSustrato químico
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Preguntas frecuentes
What is the excitation/emission maxima of resorufin?
Citations & References (14)
Citations & References
Abstract
Measurement of enzyme kinetics using a continuous-flow microfluidic system.
Journal:Anal Chem
PubMed ID:12964765
'This paper describes a microanalytical method for determining enzyme kinetics using a continuous-flow microfluidic system. The analysis is carried out by immobilizing the enzyme on microbeads, packing the microbeads into a chip-based microreactor (volume approximately 1.0 nL), and flowing the substrate over the packed bed. Data were analyzed using the
Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules.
Journal:Biochemistry
PubMed ID:12578385
'By use of a capillary electrophoresis-based procedure, it is possible to measure the activity of individual molecules of beta-galactosidase. Molecules from the crystallized enzyme as well as the original enzyme preparation used to grow the crystals both displayed a range of activity of 20-fold or greater. beta-Galactosidase molecules obtained from
Stochastic inhibitor release and binding from single-enzyme molecules.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17965235
'Inhibition kinetics of single-beta-galactosidase molecules with the slow-binding inhibitor d-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy. Inhibited and active states of beta-galactosidase could be clearly distinguished, and the large array size
Subnanoliter enzymatic assays on microarrays.
Journal:Proteomics
PubMed ID:15700241
'Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation of these assays. However, the final transfer of enzymatic assays
Microchip device for performing enzyme assays.
Journal:Anal Chem
PubMed ID:9286159
'An automated enzyme assay was performed within a microfabricated channel network. Precise concentrations of substrate, enzyme, and inhibitor were mixed in nanoliter volumes using electrokinetic flow. Reagent dilution and mixing were controlled by regulating the applied potential at the terminus of each channel, using voltages derived from an equivalent circuit