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Citations & References (14)
Invitrogen™
Resorufin β-D-Galactopyranoside
For the hydrolysis product, resorufin
| Catalog Number | Quantity |
|---|---|
| R1159 | 25 mg |
Catalog number R1159
Price (USD)
465.00
Each
Quantity:
25 mg
Price (USD)
465.00
Each
The fluorogenic β-galactosidase substrate resorufin β-D-galactopyranoside yields the hydrolysis product, resorufin, with absorption/emission maxima at 571/585 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission571/585
For Use With (Application)β-Gal Substrate
Quantity25 mg
Shipping ConditionRoom Temperature
SubstrateBeta-Gal Substrate
Detection MethodFluorescence
Emission Wavelength Range585 nm
Excitation Wavelength Range571 nm
Substrate PropertiesChemical Substrate
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Frequently asked questions (FAQs)
What is the excitation/emission maxima of resorufin?
Citations & References (14)
Citations & References
Abstract
Measurement of enzyme kinetics using a continuous-flow microfluidic system.
Journal:Anal Chem
PubMed ID:12964765
'This paper describes a microanalytical method for determining enzyme kinetics using a continuous-flow microfluidic system. The analysis is carried out by immobilizing the enzyme on microbeads, packing the microbeads into a chip-based microreactor (volume approximately 1.0 nL), and flowing the substrate over the packed bed. Data were analyzed using the
Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules.
Journal:Biochemistry
PubMed ID:12578385
'By use of a capillary electrophoresis-based procedure, it is possible to measure the activity of individual molecules of beta-galactosidase. Molecules from the crystallized enzyme as well as the original enzyme preparation used to grow the crystals both displayed a range of activity of 20-fold or greater. beta-Galactosidase molecules obtained from
Stochastic inhibitor release and binding from single-enzyme molecules.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17965235
'Inhibition kinetics of single-beta-galactosidase molecules with the slow-binding inhibitor d-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy. Inhibited and active states of beta-galactosidase could be clearly distinguished, and the large array size
Subnanoliter enzymatic assays on microarrays.
Journal:Proteomics
PubMed ID:15700241
'Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation of these assays. However, the final transfer of enzymatic assays
Microchip device for performing enzyme assays.
Journal:Anal Chem
PubMed ID:9286159
'An automated enzyme assay was performed within a microfabricated channel network. Precise concentrations of substrate, enzyme, and inhibitor were mixed in nanoliter volumes using electrokinetic flow. Reagent dilution and mixing were controlled by regulating the applied potential at the terminus of each channel, using voltages derived from an equivalent circuit