Patch-clamp detection of macromolecular translocation along nuclear pores.
AuthorsBustamante JO, Varanda WA
JournalBraz J Med Biol Res
PubMed ID9698781
'The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal ''gigaseal'' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability ... More
Extracellular Ca2+ depletion contributes to fast activity-dependent modulation of synaptic transmission in the brain.
AuthorsRusakov DA, Fine A
JournalNeuron
PubMed ID12546823
'Synaptic activation is associated with rapid changes in intracellular Ca(2+), while the extracellular Ca(2+) level is generally assumed to be constant. Here, using a novel optical method to measure changes in extracellular Ca(2+) at high spatial and temporal resolution, we find that brief trains of synaptic transmission in hippocampal area ... More
Rhod-5N as a fluorescent molecular sensor of cadmium(II) ion.
AuthorsSoibinet M, Souchon V, Leray I, Valeur B,
JournalJ Fluoresc
PubMed ID18488146
The photophysical and complexing properties of Rhod-5N (commercially available) in MOPS buffer are reported. This fluorescent molecular sensor consists of a BAPTA chelating moiety bound to a rhodamine fluorophore. Its fluorescence quantum yield is low and a drastic enhancement of fluorescence intensity upon cation binding was observed. Special attention was ... More
Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N.
AuthorsMcNamara CJ, Perry TD, Bearce K, Hernandez-Duque G, Mitchell R
JournalJ Microbiol Methods
PubMed ID15722151
Limestone and marble have been used extensively in the construction of modern and historic buildings. Microbial colonization and growth on these stone structures is common. Microbial deterioration of stone has been assessed by measuring Ca2+ released from the stone, using ion selective electrodes and titration with EDTA. In this study, ... More
Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.
AuthorsHollingworth S, Gee KR, Baylor SM,
JournalBiophys J
PubMed ID19804716
The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of the above indicators and, usually, furaptra (previously shown to rapidly ... More
Azumolene inhibits a component of store-operated calcium entry coupled to the skeletal muscle ryanodine receptor.
AuthorsZhao X, Weisleder N, Han X, Pan Z, Parness J, Brotto M, Ma J,
JournalJ Biol Chem
PubMed ID16945924
Dantrolene reduces the elevated myoplasmic Ca(2+) generated during malignant hyperthermia, a pharmacogenetic crisis triggered by volatile anesthetics. Although specific binding of dantrolene to the type 1 ryanodine receptor (RyR1), the Ca(2+) release channel of skeletal muscle sarcoplasmic reticulum, has been demonstrated, there is little evidence for direct dantrolene inhibition of ... More
Ionic calcium determination in skim milk with molecular probes and front-face fluorescence spectroscopy: simple linear regression.
AuthorsGangidi RR, Metzger LE
JournalJ Dairy Sci
PubMed ID17032997
The purpose of this study was to determine if the ionic calcium content of skim milk could be determined using molecular probes and front-face fluorescence spectroscopy. Current methods for determining ionic calcium are not sensitive, overestimate ionic calcium, or require complex procedures. Molecular probes designed specifically for measuring ionic calcium ... More
The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymatic activity.
AuthorsLiu D, Shriver Z, Godavarti R, Venkataraman G, Sasisekharan R
JournalJ Biol Chem
PubMed ID9933602
In the accompanying paper (Shriver, Z., Liu, D., Hu, Y., and Sasisekharan, R. (1999) J. Biol. Chem. 274, 4082-4088), we have shown that calcium binds specifically to heparinase I and have identified two major calcium-binding sites (CB-1 and CB-2) that partly conform to the EF-hand calcium-binding motif. In this study, ... More
Ca(2+) homeostasis in sealed t-tubules of mouse ventricular myocytes.