Thermo Scientific NTP Set, Tris buffered is a convenient set of 100 mM solutions of each of Tris buffered ATP, CTP, GTP and UTP. These extremely stable nucleotides are supplied as aqueous solutions titrated to pH 7.3-7.5 with Tris base. The nucleotides have greater than 99% purity, are free of nuclease activities and functionally tested in in vitro transcription.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of trace contaminating nucleotides, endo-, exodeoxyribonuclease and ribonuclease activities
• Higher in vitro transcription yields using Tris buffered NTPs in comparison to NTPs, titrated with NaOH
• High stability—stable for 3 years at -20°C and after multiple freeze-thaw cycles

Applications

• in vitro transcription
• RNA amplification
• siRNA synthesis
• aRNA synthesis