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Citations & References (24)
Gibco™
Blasticidin S HCl, powder
Bacterial selective antibiotic in concentration range of 50G–100μg/mL
| Catalog Number | Quantity |
|---|---|
| R21001 | 50 mg |
Catalog number R21001
Price (CLP)
275.248
Each
Quantity:
50 mg
Price (CLP)
275.248
Each
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 μg/mL depending on the cell line and 25–100 μg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
- Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome
- Used for prevention of contamination of cell cultures
- Recommended working concentration ranges from 2 to 2μg/mL depending on the cell line
- Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD (2) or bsr
Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase.
For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.
Applications: Cell Culture, Cloning, Drosophila S2 Cell Culture, High Five™ Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEukaryotic Cells, Prokaryotic Cells
ColorWhite, off-white
Concentration50 to 100 μg/mL
Culture TypeMammalian Cell Culture, Insect Cell Culture
For Use With (Application)Bacterial Selection
Product LineGibco
Quantity50 mg
Shelf LifeLot Dependent
FormPowder
Product TypeAntibiotic
SterilitySterile
With AdditivesHEPES
Unit SizeEach
Contents & Storage
Storage conditions: -5 to -30°C
Shipping conditions: room temperature
Shelf life: Lot Dependent
Shipping conditions: room temperature
Shelf life: Lot Dependent
Frequently asked questions (FAQs)
Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?
What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?
What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?
What is the optimal pH of low salt LB for LB + blasticidin plates?
How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?
Citations & References (24)
Citations & References
Abstract
Suppression of Ras-mediated tumorigenicity and metastasis through inhibition of the Met receptor tyrosine kinase.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11535809
'Mutations in the Ras family of GTP binding proteins represent one of the most frequently observed genetic alterations in human cancers. We and others have recently demonstrated that expression of Met, the tyrosine kinase receptor for hepatocyte growth factor/scatter factor (HGF/SF), is significantly up-regulated in Ras-transformed cells. Because HGF/SF-Met signaling
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
Journal:J Biol Chem
PubMed ID:7730310
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type
West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.
Journal:J Virol
PubMed ID:16415006
'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile
Presenilin 1 mutations activate gamma 42-secretase but reciprocally inhibit epsilon-secretase cleavage of amyloid precursor protein (APP) and S3-cleavage of notch.
Journal:J Biol Chem
PubMed ID:12119298
'The presenilin 1 (PS1) and presenilin 2 (PS2) proteins are necessary for proteolytic cleavage of the amyloid precursor protein (APP) within its transmembrane domain. One of these cleavage events (termed gamma-secretase) generates the C-terminal end of the Abeta-peptide by proteolysis near residue 710 or 712 of APP(770). Another event (termed
Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.
Journal:Nucleic Acids Res
PubMed ID:15520459
'Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell