Hippurate Disk

Rapidly detect the hydrolysis of sodium hippurate by β-hemolytic streptococci, Gardnerella vaginalis, and Campylobacter jejuni using a reagent-impregnated Thermo Scientific™ Remel™ Hippurate Disk. Hwang and Ederer employed the ninhydrin reagent in a rapid method to detect hippurate hydrolysis by the end product glycine¹. Jolly later outlined minimal criteria for identification of G. vaginalis including hippurate hydrolysis². In 1989, Cacho et al. further evaluated the use of the hippurate disk in the identification of Campylobacter spp³.

Rapidly detect the hydrolysis of sodium hippurate by β-hemolytic streptococci, Gardnerella vaginalis, and Campylobacter jejuni using a reagent-impregnated Hippurate Disk. The hippurate hydrolysis test determines the ability of Group B streptococci, as well as other bacteria, to enzymatically hydrolyze sodium hippurate^4,5.

• Ready to use: Reagent-impregnated disk
• Rapid test: Results are obtained within 30 minutes.
• Easy to identify: A condensation reaction occurs with hydrindantin, ammonia, and residual ninhydrin to produce the final purple colored complex for a positive reaction and light gray color for a negative reaction.

Hippuric acid is hydrolyzed by the enzyme hippuricase to glycine and benzoic acid. Ninhydrin evokes a five-step reaction beginning with the deamination of glycine to form hydrindantin (a reduced form of ninhydrin), carbon dioxide, and ammonia.

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.