Thermo Scientific™

E. coli Screen w/Kovacs

Catalog number: R211347
Have Questions?
Thermo Scientific™

E. coli Screen w/Kovacs

Catalog number: R211347
Have Questions?

Presumptively identify Escherechia coli using the Thermo Scientific™ Remel™ E. coli Screen w/Kovacs.

In 1981, Godsey reported the utility of enzyme activity profiles for rapid identification of Enterobacteriaceae1 . He observed that E.coli and Shigella sonnei can be differentiated from other species by detecting β-glucuronidase. Trepeta and Edberg further demonstrated that detecting β-glucuronidase along with oxidase, indole and lactose fermentation can be used for rapid, cost-effective identification E. coli2,3.

 
Catalog Number
R211347
Unit Size
Each
Quantity
50 Tests/Kit
Price (USD)
Full specifications
DescriptionE. coli Screen w/Kovacs
Detectable AnalytesE. coli
TypeE. coli Testing Kit
For Use With (Application)PGUA/Indole Enzyme Tests
Quantity50 Tests/Kit
Unit SizeEach
Showing 1 of 1
Catalog NumberSpecificationsUnit SizeQuantityPrice (USD)
R211347Full specifications
Each50 Tests/KitRequest A Quote
DescriptionE. coli Screen w/Kovacs
Detectable AnalytesE. coli
TypeE. coli Testing Kit
For Use With (Application)PGUA/Indole Enzyme Tests
Quantity50 Tests/Kit
Unit SizeEach
Showing 1 of 1

Escherechia coli is the most commonly found bacteria in clinical laboratory settings involving a wide range of diseases ranging from infected urinary tract to gram negative sepsis4. E.coli screen is medium recommended for presumptive identification of E. coli.

  • Ready to use: E. coli screen tubes and Kovac’s Indole Reagent.
  • Easy interpretation: Visible colour change

E. coli Screen w/Kovacs contains E. coli Screen Tubes and Kovac’s Indole Reagent. The E. coli screen tubes contain Columbia Agar, tryptophane and ρ-nitrophenyl β-D-glucopyranosiduronic acid (PGUA). PGUA is a colorless compound that is hydrolyzed by β-glucuronidase to form nitrophenyl, a yellow compound. In the indole test, tryptophanase enzyme attacks the side chain of tryptophan and leaves an aromatic ring in the form of indole. This reaction occurs as a condensation process that is formed by an acid splitting of the protein molecule. The ρ-dimethylaminobenzaldehyde in Kovacs reagent then combines with indole to form a red color complex5,6.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

For in vitro diagnostic use.
  1. Godsey, J.H., M.R. Matteo, D. Shen, G. Tolman, and J.R. Gohlke. 1981. J. Clin. Microbiol. 13:483-490.
  2. Edberg, S.C. and R.W. Trepeta. 1983. J. Clin. Microbiol. 18: 1287-1291.
  3. Trepata, R.W. and S.C. Edberg. 1984. J. Clin. Microbiol. 19:172-174.
  4. Koneman, E.W., S.D. Allen, W.M. Janda, P.C. Schreckenberger, and W.C. Winn, Jr. 1992. Color Atlas and Textbook of Diagnostic Microbiology. 4th J.B. Lippincott Co., Philadelphia, P.A.
  5. MacFaddin, J.F. 1980. Biochemical Tests for Identification of Medical Bacteria. 2nd Williams & Wilkins, Baltimore, M.D.
  6. Blazevic, D.J. and G.M. Ederer. 1975. Principles of Biochemical Tests in Diagnostic Microbiology. John Wiley & Sons, New York, NY.

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