Enterococcus/Group A Strep Screen

Presumptively identify Group A Streptococci and Enterococci using Thermo Scientific™ Remel™ Enterococcus/Group A Strep Screen. Rochaix first demonstrated the value of esculin hydrolysis in the identification of enterococci¹. In 1970, Mulczyk and Szewczuk described the L-pyroglutamic acid β-naphthylamide (PYR) hydrolysis test². These combinations of tests were useful in the identification of Streptococcus pyogenes, Enterococcus species, and other gram-positive cocci³.

Presumptively identify Group A Streptococci and Enterococci using Enterococcus/Group A Strep Screen.

• Easy to store - No special storage required
• Easy to interpret - With visual inspection - the color change in the medium to dark brown indicates a positive esculin reaction and after addition of two drops of Cinnamaldehyde Reagent to the tube, the color changes to red indicating a positive PYR test.

This medium contains esculin compound which is hydrolyzed enzymatically at the β-glucose linkage to yield two products – esculetin and glucose. Esculetin reacts with the ferric ammonium citrate present in the medium to form a phenolic iron complex which is brown-black in color.

L-pyroglutamic acid β-naphthylamide (PYR) serves as the substrate for the detection of pyrrolidonyl peptidase. Following substrate hydrolysis by the peptidase enzyme, the resulting β-naphthylamine produces a red color upon the addition of ρ-dimethyl-aminocinnamaldehyde (Cinnamaldehyde Reagent).