Detect fungal elements in clinical specimens by microscopic examination using Thermo Scientific™ Remel™ 10% Potassium Hydroxide preparation. The Laboratory Handbook of Medical Mycology recommends the use of 10% KOH for direct detection of fungal elements1.The KOH preparation, when positive, provides valuable clues as to the etiological agent of infection. McGowan reported the use of 10% KOH but warned that a lack of contrast between fungal elements and clinical material may occur2. Hence, for improved visibility phase contrast microscopy is recommended.
Directly detect fungal elements in clinical specimens by microscopic examination using 10% Potassium Hydroxide preparation. KOH is a strong base.Ready for Use: Convenience of a prepared reagent.Quick and inexpensive detection of fungal elements in clinical specimensKOH acts to disrupt cellular sheets or clumps of proteinaceous debris that may be present and clears the specimen for easier fungal detection3. The addition of glycerol to the KOH solution reduces the amount of precipitation that forms in the reagent containers and prevents KOH preparations from drying4.Not all products are available for sale in all territories. Please inquire.
- McGinnis, M.R. 1980. Laboratory Handbook of Medical Mycology. Academic Press, New York, N.Y.
- McGowan, K.L. 1987. Clin. Microbiol. News. 9:5.
- Elder, B.L. and G.D. Roberts. 1986. Lab. Med. 17: 591-596.
- Haley, L.D. and C.S. Callaway. 1978. Laboratory Methods in Medical Mycology. 4th ed. U.S. Dept. of H.H.S. and CDC, Atlanta, GA.