Quant-it™ RiboGreen Reagent and RNA Assay Kit
Quant-it™ RiboGreen Reagent and RNA Assay Kit
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Invitrogen™

Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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Catalog NumberProduct TypeQuantitation Range
R32700RediPlate 96 RiboGreen RNA Kit3 to 200 ng
R11490Quant-iT RiboGreen RNA Kit1 to 200 ng
R11491Quant-iT RiboGreen RNA Reagent1 to 200 ng
Catalog number R32700
Price (USD)/ Each
291.65
Online exclusive
308.00 
Save 16.35 (5%)
-
Add to cart
Product Type:
RediPlate 96 RiboGreen RNA Kit
Quantitation Range:
3 to 200 ng
Price (USD)/ Each
291.65
Online exclusive
308.00 
Save 16.35 (5%)
Add to cart
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Catalog numberR32700
Price (USD)/ Each
291.65
Online exclusive
308.00 
Save 16.35 (5%)
-
Add to cart
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
Quantitation Range3 to 200 ng
Product LineRIBOGREEN, RediPlate
Product TypeRediPlate 96 RiboGreen RNA Kit
Quantity1 kit
Shipping ConditionRoom Temperature
Unit SizeEach