Invitrogen™

RediPlate™ 96 EnzChek™ Serine/Threonine Phosphatase Assay Kit

Catalog number:  R33700

Related applications:

Protein Assays and Analysis

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Description

The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Phosphatase Assay
Excitation/Emission: 358⁄452
Shipping Condition: Room Temperature
Substrate Type: Phosphatase Substrate
Substrate Properties: Chemical Substrate
Substrate: DiFMUP
Target Enzyme: Serine⁄Threonine Phosphatases
Technique: Fluorescence Intensity
Product Line: EnzChek®, RediPlate™
Detection Method: Fluorescent
Label or Dye: DiFMU
For Use With (Equipment): Microplate Reader

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.