RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit

Catalog number: R33700

Molecular Probes™  Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: DiFMUP
Technique: Fluorescence Intensity
Assay Type: Phosphatase Assay
Label or Dye: DiFMU
Product Size: 1 x 96 well plate
Target Enzyme: Serine⁄Threonine Phosphatases
Substrate Type: Phosphatase Substrate
Detection Method: Fluorescent
Substrate Properties: Chemical Substrate
For Use With (Equipment): Microplate Reader
Excitation⁄Emission (nm): 358⁄452
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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