RediPlate™ 96 EnzChek™ Serine/Threonine Phosphatase Assay Kit

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The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.
For Research Use Only. Not for use in diagnostic procedures.


Assay Type: Phosphatase Assay
Detection Method: Fluorescent
Excitation⁄Emission (nm): 358⁄452
For Use With (Equipment): Microplate Reader
Label or Dye: DiFMU
Product Line: EnzChek®, RediPlate™
Product Size: 1 x 96 well plate
Substrate: DiFMUP
Substrate Properties: Chemical Substrate
Substrate Type: Phosphatase Substrate
Target Enzyme: Serine⁄Threonine Phosphatases
Technique: Fluorescence Intensity
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols