Invitrogen™
RediPlate™ 96 EnzChek™ Serine/Threonine Phosphatase Assay Kit
Catalog number: R33700
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Description
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Assay Type: | Phosphatase Assay |
|---|---|
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 358⁄452 |
| For Use With (Equipment): | Microplate Reader |
| Label or Dye: | DiFMU |
| Product Line: | EnzChek®, RediPlate™ |
| Product Size: | 1 x 96 well plate |
| Substrate: | DiFMUP |
| Substrate Properties: | Chemical Substrate |
| Substrate Type: | Phosphatase Substrate |
| Target Enzyme: | Serine⁄Threonine Phosphatases |
| Technique: | Fluorescence Intensity |
| Shipping Condition: | Room Temperature |
Contents & storage
Store in freezer (-5 to -30°C) and protect from light.
