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Invitrogen™
NucRed™ Live 647 ReadyProbes™ Reagent
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Description
NucRed® Live 647 ReadyProbes® Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells.• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited at 638 nm when bound to DNA, with an emission maximum at 686 nm
See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging
Cell imaging applications
The far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red®.
Suggestions for use
• NucRed® Live 647 ReadyProbes® Reagent may be added directly to cells in full media, or buffer solutions.
• In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity.
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• It is detected through a far-red filter, such as a Cy®5 filter, or a Cy®5 Light Cube for EVOS® imaging systems.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Color: | Far-Red |
|---|---|
| For Use With (Equipment): | Confocal Microscope, Flow Cytometer, Fluorescence Microscope |
| Cell Permeability: | Cell-Permeant |
| Sub-Cellular Localization: | Nucleus |
| Detection Method: | Fluorescent |
| Emission Class: | Visible |
| Excitation Class: | Visible |
| Excitation⁄Emission (nm): | 638⁄686 |
| Form: | Liquid |
| Product Line: | NucRed™, ReadyProbes® |
| Product Size: | 1 kit |
| Quantity: | 6 dropper bottle(s) |
Contents & storage
6 × 2.5 mL dropper bottles
Store at ≤ 25°C
Store at ≤ 25°C
