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Invitrogen™

NucRed™ Live 647 ReadyProbes™ Reagent

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Description

NucRed Live 647 ReadyProbes Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited at 638 nm when bound to DNA, with an emission maximum at 686 nm

See other ReadyProbes reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
The far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red.

Suggestions for use
• NucRed Live 647 ReadyProbes Reagent may be added directly to cells in full media, or buffer solutions.
• In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity.
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• It is detected through a far-red filter, such as a Cy5 filter, or a Cy5 Light Cube for EVOS imaging systems.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Far-Red
For Use With (Equipment): Confocal Microscope, Flow Cytometer, Fluorescence Microscope
Cell Permeability: Cell-Permeant
Sub-Cellular Localization: Nucleus
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 638⁄686
Form: Liquid
Product Line: NucRed™, ReadyProbes®
Product Size: 1 kit
Quantity: 6 dropper bottle(s)

Contents & storage

6 × 2.5 mL dropper bottles

Store at ≤ 25°C

Documents

Manuals & protocols