NucRed™ Live 647 ReadyProbes™ Reagent

Description

NucRed® Live 647 ReadyProbes® Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited at 638 nm when bound to DNA, with an emission maximum at 686 nm

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
The far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red®.

Suggestions for use
• NucRed® Live 647 ReadyProbes® Reagent may be added directly to cells in full media, or buffer solutions.
• In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity.
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• It is detected through a far-red filter, such as a Cy®5 filter, or a Cy®5 Light Cube for EVOS® imaging systems.

Figures

Specifications

Color:	Far-Red
For Use With (Equipment):	Confocal Microscope, Flow Cytometer, Fluorescence Microscope
Cell Permeability:	Cell-Permeant
Sub-Cellular Localization:	Nucleus
Detection Method:	Fluorescent
Emission Class:	Visible
Excitation Class:	Visible
Excitation⁄Emission (nm):	638⁄686
Form:	Liquid
Product Line:	NucRed™, ReadyProbes®
Product Size:	1 kit
Quantity:	6 dropper bottle(s)

Contents & storage