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NucRed® Live 647 ReadyProbes® Reagent

Catalog number: R37106

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

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Description

NucRed® Live 647 ReadyProbes® Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited at 638 nm when bound to DNA, with an emission maximum at 686 nm

See other ReadyProbes® reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
The far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red®.

Suggestions for use
• NucRed® Live 647 ReadyProbes® Reagent may be added directly to cells in full media, or buffer solutions.
• In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity.
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• It is detected through a far-red filter, such as a Cy®5 filter, or a Cy®5 Light Cube for EVOS® imaging systems.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Far-Red
For Use With (Equipment): Confocal Microscope, Flow Cytometer, Fluorescence Microscope
Cell Permeability: Cell-Permeant
Sub-Cellular Localization: Nucleus
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 638⁄686
Form: Liquid
Product Size: 1 kit
Quantity: 6 dropper bottle(s)

Contents & storage

6 × 2.5 mL dropper bottles

Store at ≤ 25°C

Documents

Manuals & protocols