Image RNA at single-molecule sensitivity and single-cell resolution with ViewRNA Cell Plus Assay.

ActinGreen™ 488 ReadyProbes™ Reagent

Catalog number:  R37110

 Related applications:

Cell Structure

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


ActinGreen™ 488 ReadyProbes® Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. Like other ReadyProbes® reagents, the ActinGreen™ 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 495/518 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area

See other ReadyProbes® reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 dye provides green fluorescence of unparalleled brightness and photostability.

Suggestions for use
• ActinGreen™ 488 reagent may be added directly to fixed cells in full media or buffer solutions.
• In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinGreen™ 488 dye is excited with a maximum at 495 nm and has an emission maximum at 518 nm. It is detected through standard GFP and FITC filters.
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: Alexa Fluor™ 488
Sub-Cellular Localization: Cytoskeleton
Color: Green
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 495⁄518
For Use With (Equipment): Confocal Microscope, Floid® Cell Imaging System, Flow Cytometer, Fluorescence Microscope
Form: Liquid
Product Line: ActinGreen™, ReadyProbes®
Product Size: 1 kit

Contents & storage

2 × 2.5 mL dropper bottles

Store at ≤ 25°C.


Manuals & protocols