Invitrogen™

Propidium Iodide Ready Flow™ Reagent

Catalog number:  R37169

Related applications:

Flow Cytometry

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Description

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 535/617 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh. or pipette
• Rapid staining of dead cells without wash steps

Propidium Iodide Ready Flow Reagent is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

Propidium iodide is membrane impermeant and therefore excluded from viable cells. It binds to DNA by intercalating between the bases, thereby binding both DNA and RNA. Propidium iodide is commonly excited with the 488 nm laser, but others such as the 532 and 561 nm lasers are also suited for PI excitation.

Specifications

Cell Permeability: Cell-Impermeant
Excitation/Emission: 535⁄617
Product Line: Ready Flow™
Sub Cellular Localization: Nucleus
Color: Red

Contents & storage

2 dropper bottles, store at room temperature