Invitrogen™

TO-PRO™3 Ready Flow™ Reagent

Catalog number:  R37170

Related applications:

Cell Analysis

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Description

TO-PRO™3 Ready Flow™ Reagent is a bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC).

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Rapid staining of dead cells without wash steps

TO-PRO 3 Ready Flow Reagent is a ready-to-use, direct-to-sample dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops form the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

TO-PRO 3 Ready Flow Reagent is membrane impermeant and therefore excluded from viable cells. It binds selectively to double-stranded DNA. This dye has excitation/emission maxima of 642/661 nm.

Specifications

Color: Far-Red
Cell Permeability: Cell-Impermeant
Excitation/Emission: 642⁄661
Sub Cellular Localization: Nucleus
Product Line: Ready Flow™, TO-PRO™3

Contents & storage

2 dropper bottles, store at room temperature