ReadyProbes™ F-Actin Labeling Reagents
ReadyProbes™ F-Actin Labeling Reagents
ReadyProbes™ F-Actin Labeling Reagents
Invitrogen™

ReadyProbes™ F-Actin Labeling Reagents

ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.
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Número de catálogoTipo de coloranteIntervalo de longitud de onda de excitación
R37178Alexa Fluor™ 568580/603
R37110Alexa Fluor™ 488495/518
R37112TRITC (isotiocianato de tetrametilrodamina)540/565
R37179Alexa Fluor™ Plus 647658/675
Número de catálogo R37178
Precio (MXN)
-
Tipo de colorante:
Alexa Fluor™ 568
Intervalo de longitud de onda de excitación:
580/603

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 ActinGreen (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine F-actin probe (Cat. No. R415) packaged in a dropper bottle.

ActinRed 568 ReadyProbes Reagent is our high-affinity F-actin probe conjugated to our bright, red-fluorescent Alexa Fluor 568 dye. This dye is packaged in an easy to use dropper-optional bottle, allowing end-users to choose either to pipet out of the tube using the classic cap, or utilize the dropper bottle tip for typical ReadyProbes workflows.

ActinRed 647 ReadyProbes Reagent leverages our high-affinity F-actin probe conjugated to our bright Far-Red Alexa Fluor 647 Plus dye, offering enhanced brightness vs. classic Alexa Fluor 647 F-actin probe conjugates, in an easy to use format. ActinRed 647 ReadyProbes Reagent should be stored from 2-8°C for long-term, and also offers the option to leverage pipetting, if desired, via the dropper-optional cap.

These ready-to-use solutions combine superior brightness with exceptional F-actin staining. ReadyProbes actin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Permeabilidad celularFixed Cells
ColorRed
ConcentraciónReady-to-use
Tipo de entregaAdd 2 drops (80 μL) per 1 mL of medium
Método de detecciónFluorescence
Tipo de coloranteAlexa Fluor™ 568
Emisión603 nm
Excitación580 nm
Intervalo de longitud de onda de excitación580/603
Para utilizar con (aplicación)Fixed Cell Imaging, High Content Screening, Fluorescence Microscopy
Para utilizar con (equipo)Confocal Microscope, EVOS™, CellInsight™ Cell Imaging Instruments, Fluorescence Microscope, Flow Cytometer
FormularioReady-to-use aqueous solution
FormatoDual-use Dropper Bottle
IncluyeDropper Tip, Dropper Cap
Nº de pruebas75 Tests
Línea de productosActinRed, ReadyProbes
Cantidad1 Dual-use bottle
ReactividadF-actin
SinónimoPhalloidin
ObjetivoF-actin Cytoskeleton
TécnicaCell Paint Assay
Volumen (métrico)6 mL
Tipo de etiquetaFluorescent Dye
Tipo de productoCell Labeling
Sub Cellular LocalizationCytoskeleton
Unit SizeEach
Contenido y almacenamiento
•1 Dual-use Bottle
•Store at 15°C to 30°C
•Protect from light
•Do not freeze

Preguntas frecuentes

Do you offer alternative package sizes for phalloidin or ReadyProbes reagents?

Alternative package sizes may be available through a custom request process. Contact Thermo Fisher Scientific customer support or your sales representative to discuss available options for the specific reagent and quantity needed.

Can I use conjugated phalloidin label probes on organoids?

Yes. Conjugated phalloidin labeling probes can be used with organoids when F-actin is accessible after fixation and permeabilization. Because organoids are thicker than monolayer cell cultures, protocol optimization may be needed, including permeabilization conditions, probe incubation time, and probe concentration.

Are conjugated phalloidin reagents antibodies?

No, Phalloidin is not an antibody. It is a bicyclic peptide toxin originally isolated from the Amanita phalloides mushroom and is used to bind and label F-actin in fixed and permeabilized samples.

Are ReadyProbes ActinRed and ActinGreen reagents stable at room temperature?

Yes. ReadyProbes ActinRed and ActinGreen reagents are designed for storage between 15°C and 30°C. Store the reagents according to the product instructions and protect them from light.

Can I use conjugated phalloidin reagents with IHC, FFPE sections, or cryosections?

Yes. Fluorescent phalloidin can be used with appropriately prepared fixed and permeabilized tissue sections, especially cryosections. FFPE processing and deparaffinization can include organic solvents such as xylene or acetone, which may alter F-actin and reduce phalloidin binding. If paraffin sections show weak or no signal, consider cryosections or an anti-actin antibody.