ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

Citations & References (15)

Invitrogen™

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

ReadyProbes™細胞生存率イメージングキット（Blue/Green）はすぐに使用可能なアッセイで、細胞の生存率を迅速かつ簡単に測定できます。室温で安定したNucBlue™ Live試薬（Hoechst 33342）とNucGreen™ Dead試薬を1詳細を見る

製品番号（カタログ番号）	数量
R37609	1 kit

製品番号（カタログ番号） R37609

価格（JPY）

51,200

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1 kit

ReadyProbes™細胞生存率イメージングキット（Blue/Green）はすぐに使用可能なアッセイで、細胞の生存率を迅速かつ簡単に測定できます。室温で安定したNucBlue™ Live試薬（Hoechst 33342）とNucGreen™ Dead試薬を1 mLの細胞増殖培地にそれぞれ2滴加え、総細胞数と死細胞数を計数することで生存率を測定します。NucBlue™ Live試薬は、すべての細胞の核を染色して標準的なDAPIフィルターで検出できます。NucGreen™ Dead試薬は、細胞膜の完全性が損なわれた細胞の核のみを染色し、標準的なFITC/GFP（緑）フィルターセットを使用して検出します。このキットは、蛍光顕微鏡、蛍光マイクロプレートリーダー、およびフローサイトメトリーに適しています。

NucBlue™ Live試薬： すべての細胞の核を染色します。標準的なDAPIフィルターを使用して検出します（励起／発光極大：360/460 nm）
NucGreen™ Dead試薬： 細胞膜に損傷のある死細胞の核のみを染色します。標準的な FITC/GFP（緑）フィルターセットを使用して検出します（励起／発光極大：504/523 nm）
その他の細胞染色用ReadyProbes™試薬を見る
細胞生存率に関するその他のアッセイの詳細を見る

使用方法のヒント
• NucBlue™ Liveおよび NucGreen™ Dead試薬は、完全増殖培地または適合する緩衝液で細胞に直接添加できます。
• ほとんどの場合、2滴/mLかつインキュベーション時間5∼30分では明るい核染色を示しますが、一部の細胞種、条件、およびアプリケーションでは最適化が必要な場合があります。このような場合は、最適な染色強度が得られるまで、加える量を増減します。

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプ哺乳類細胞、真核細胞

概要ReadyProbes Cell Viability Imaging Kit, Blue/Green

検出法蛍光

染色剤タイプ3 x 2.5 mL NucBlue™ Live、3 x 2.5 mL NucGreen™ Dead

フォーマットドロッパーボトル

数量1 kit

出荷条件室温

色青色、緑色

Emission360/504

Excitation Wavelength Range460, 523 nm

使用対象（アプリケーション）細胞の生存率の測定

使用対象 (装置)蛍光顕微鏡

製品ラインReadyProbes

製品タイプCell Viability Imaging Kit

Unit SizeEach

組成および保存条件

2℃∼8℃または室温で保管してください。

よくあるご質問（FAQ）

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between NucBlue Live reagents stain and NucGreen Dead reagents stain?

The NucBlue Live reagent stains the nuclei of all cells, while NucGreen Dead reagent stains only the nuclei of dead cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (15)

引用および参考文献

Abstract

In vitro studies with renal proximal tubule cells show direct cytotoxicity of Androctonus australis hector scorpion venom triggered by oxidative stress, caspase activation and apoptosis.

Authors:Saidani C, Hammoudi-Triki D, Laraba-Djebari F, Taub M,

Journal:Toxicon

PubMed ID:27470530

'Scorpion envenomation injures a number of organs, including the kidney. Mechanisms proposed to explain the renal tubule injury include direct effects of venom on tubule epithelial cells, as well as indirect effects of the autonomic nervous system, and inflammation. Here, we report direct effects of Androctonus australis hector (Aah) scorpion ... More

Fluorescence and Cytotoxicity of Cadmium Sulfide Quantum Dots Stabilized on Clay Nanotubes.

Authors:Stavitskaya AV, Novikov AA, Kotelev MS, Kopitsyn DS, Rozhina EV, Ishmukhametov IR, Fakhrullin RF, Ivanov EV, Lvov YM, Vinokurov VA

Journal:Nanomaterials (Basel)

PubMed ID:29857546

'Quantum dots (QD) are widely used for cellular labeling due to enhanced brightness, resistance to photobleaching, and multicolor light emissions. CdS and Cd'

Chemobrionic structures in tissue engineering: self-assembling calcium phosphate tubes as cellular scaffolds.

Authors:Hughes EAB, Chipara M, Hall TJ, Williams RL, Grover LM

Journal:Biomater Sci

PubMed ID:31830151

'A diverse range of complex patterns and mineralised hierarchical microstructures can be derived from chemobrionic systems, with formation driven by complex reaction-diffusion mechanisms far from thermodynamic equilibrium. In these experiments, self-assembling calcium phosphate tubes are generated using hydrogels made with 1 M calcium solutions layered with solutions of dibasic sodium ... More

An evolutionary perspective on the role of mesencephalic astrocyte-derived neurotrophic factor (MANF): At the crossroads of poriferan innate immune and apoptotic pathways.

Authors:Sereno D, Müller WEG, Bausen M, Elkhooly TA, Markl JS, Wiens M,

Journal:Biochem Biophys Rep

PubMed ID:28955781

'The mesencephalic astrocyte-derived neurotrophic factor (MANF) belongs to a recently discovered family of neurotrophic factors. MANF can be secreted but is generally resident within the endoplasmic reticulum (ER) in neuronal and non-neuronal cells, where it is involved in the ER stress response with pro-survival effects. Here we report the discovery ... More

A novel method to understand tumor cell invasion: integrating extracellular matrix mimicking layers in microfluidic chips by

Authors:Eslami Amirabadi H, SahebAli S, Frimat JP, Luttge R, den Toonder JMJ,

Journal:Biomed Microdevices

PubMed ID:29038872

'A major challenge in studying tumor cell invasion into its surrounding tissue is to identify the contribution of individual factors in the tumor microenvironment (TME) to the process. One of the important elements of the TME is the fibrous extracellular matrix (ECM) which is known to influence cancer cell invasion, ... More

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