Invitrogen™

ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Catalog number: R37610
Invitrogen™

ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Catalog number: R37610
Catalog Number
R37610
Unit Size
1 kit
Price (USD)
Price: 312.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R376101 kit
Price: 312.00
Your Price:
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Product Overview
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Additional Information
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Recommendations
ReadyProbes™ Cell Viability Imaging Kit (Blue/Red) is a ready-to-use kit that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue™ Live reagent (Hoechst 33342) and propidium iodide to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set. This kit is appropriate for fluorescence microscopy, fluorescence micro plate readers, and flow cytometry.

NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)
Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617 nm)
See other ReadyProbes™ reagents for cell staining
Learn more about other assays for cell viability

Suggestions for use
• NucBlue™ Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Mammalian Cells, Eukaryotic Cells
Color
Blue, Red
Detection Method
Fluorescence
For Use With (Application)
Determining Viability of Cells
For Use With (Equipment)
Fluorescence Microscope
Product Type
Cell Viability Imaging Kit
Dye Type
3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide
Format
Dropper Bottles

Contents & Storage

Store at 2° to 8°C, or at room temperature.

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