Invitrogen™

ReadyProbes™ Tissue Autofluorescence Quenching Kit

Catalog number: R37630
Have Questions?
Invitrogen™

ReadyProbes™ Tissue Autofluorescence Quenching Kit

Catalog number: R37630
Have Questions?
Catalog Number
R37630
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R376301 kit
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays. The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.

The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio. Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature. This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
1 Kit
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Product Line
ReadyProbes™
Product Type
Blocking Reagent

Contents & Storage

A kit containing 5 mL each ready-to-use (1X) solutions of reagent A, B, and C in three clear HDPE bottles.
  • Store at 2–8°C.
  • When stored as directed, product can be used for 3 months after receipt.One kit is sufficient to tre

    Figures

    Documents & Downloads

    Certificates

      Frequently asked questions (FAQs)

      Citations & References

      Search citations by name, author, journal title or abstract text