Use of gram staining kit helps in differentiating gram-positive organisms from gram-negative organisms.

• Ready-to-use: Liquid reagents supplied in 250mL bottles
• Ease of identification: As it stains the gram positive organisms as purple and gram-negative organisms as red.
• Rapid results: 5-10 mins for the staining.

The gram staining kit consists of 4 bottles containing 250 mL of Gram Crystal Violet, Safranin, Gram Iodine and Gram Decolorizer each.

Crystal Violet, a basic dye, is the primary stain taken up by all bacteria due to its ability to rapidly permeate the cell wall. It stains the protoplast of bacteria purple. The potassium-iodine mixture, in gram iodine, is the mordant which complexes with the primary stain in the cell. In gram-positive cells, the crystal violet-iodine complex is trapped in the cell due to a decrease in cell wall permeability caused by alcohol dehydration. Gram-positive cells are stained purple.

In gram-negative cells, the complex is removed by the Decolorizer due to an increase in permeability caused by solubility of the lipids in alcohol.

The counterstain used must be a contrasting color to the primary stain and, in this case, it is Safranin which stains gram-negative cells red.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.