Methanol

Thermo Scientific™ Remel™ Absolute Methanol is used with the modified acid-fast stain procedure for detection of Cryptosporidium in clinical specimens. Ma and Soave recommended a modified acid-fast staining procedure because of its simplicity and specificity for detecting Cryptosporidium¹. Garcia recommended a more recent modification of the Kinyoun carbolfuchsin stain that is used to detect Cryptosporidium oocysts in clinical specimens ^2,3. Absolute Methanol is a fixative used to preserve the morphology of the cells and aids in detection of Cryptosporidium oocysts.

Cryptosporidium is an intestinal protozoan parasite well known for causing diarrhoea in animals and has more recently been recognized for causing human disease. Oocysts are spherical 4-6μm in diameter, and stain pink to red to purple with Kinyoun carbolfuchsin stain. Most will contain several dark staining granules.

• Ready-to-use – Prepared reagents
• Easy-to-store – Store in its original container at room temperature (20-25°C) until further use
• Easy to interpret – Oocysts are spherical and stain pink to red to purple with Kinyoun carbolfuchsin stain whereas yeast cells which are morphologically like Cryptosporidium, stain blue or green depending on the counterstain used.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.