Phenol Red Broth Base (Dehydrated)

Determine fermentation reactions of bacteria with Thermo Scientific™ Remel™ Phenol Red Broth Base (Dehydrated). Vera recommended using casein peptone in fermentation test media because of its high growth-promoting qualities¹. It was reported that peptone could be used with phenol red as the pH indicator in fermentation tests.

Phenol Red Broth Base is a basal medium prepared with phenol red as an indicator which changes the medium to yellow when acid is produced. Carbohydrates may be added to the broth base at a final concentration of 1%. A Phenol Red Broth Base control tube is used as a negative control for fermentation studies. A Durham tube may be used to allow detection of gas production.

• Can be used in fermentation studies of pure cultures of many species of bacteria due to high growth-promoting qualities of casein peptone.
• Recommended by Association of Official Analytical Collaboration (AOAC) International and the American Public Health Association (APHA)^2-5
• Easy to interpret because of fermentation reaction and gas production. The medium is yellow when there is acid production and red-orange color when there is no fermentation. Bubbles present in the Durham tube indicate gas production.

Phenol Red Broth Base contains casein peptone that has high growth-promoting qualities. Phenol red is the indicator which determines the reaction by a color change. Sodium chloride supplies essential electrolytes and maintains osmotic equilibrium of the medium.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.