Remel™ m Enterococcus Agar

Selectively isolate and enumerate enterococci by membrane filtration or by direct plating using Thermo Scientific™ Remel™ m-Enterococcus Agar (Dehydrated). In 1957, Slanetz and Bartley reported that m-Enterococcus Agar was superior to other media for detecting and enumerating fecal streptococci by the membrane filtration technique¹. Saraswat et al. used m-Enterococcus Agar to select enterococci in dried foods, including non-fat dry milk². m-Enterococcus Agar is recommended in Standard Methods for the Examination of Water and Wastewater and Standard Methods for the Examination of Dairy Products.

The Enterococcus count is used as an indicator of sanitary quality in water and dairy products^3,4.

Use m-Enterococcus Agar for selective isolation and enumeration of enterococci by membrane filtration or by direct plating.

• Recommended in Standard Methods for the Examination of Water and Wastewater and Standard Methods for the Examination of Dairy Products
• Selective medium – Sodium azide inhibits gram-negative organisms
• Easy-to-interpret – Enterococci form pink to red colonies of 0.5 – 3 mm in diameter

The medium contains casein and soy peptones that provide nitrogenous compounds, amino acids and peptides necessary for bacterial growth. Yeast extract supplies B-complex vitamins which are essential for bacterial metabolism. Dextrose provides a ready source of energy. Dipotassium phosphate acts as a buffer. Sodium azide is a selective agent that inhibits gram-negative organisms. Triphenyltetrazolium chloride (TTC) is the dye that serves as an indicator of bacterial growth and agar is the solidifying agent. TTC gets reduced to soluble formazan inside the bacterial cell resulting in the production of red colonies.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.