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Detect and identify Chlamydia trachomatis directly from urethral and endocervical specimens with the non-culture immunofluorescence Chlamydia Trachomatis FA Direct Test Kit.
Detect and identify Chlamydia trachomatis directly from urethral and endocervical specimens by immunofluorescence with Thermo Scientific™ Remel™ PathoDx™ Chlamydia Trachomatis FA Direct Test Kit. Chlamydiae are the most common etiologic agents in sexually transmitted diseases1. C. trachomatis is currently recognized as the cause of cervicitis and pelvic inflammatory disease (PID) in female2,3,4. Chlamydiae are propagated in tissue culture with host cells that are chemically treated for enhanced susceptibility to infection5.
Currently, several diagnostic procedures are available for detecting C. trachomatis in clinical specimens. These include enzyme immunoassay, inoculation of cell cultures followed by staining with iodine, Giemsa, or fluorescein-conjugated antibodies, and direct detection of chlamydial antigens in clinical samples by immunofluorescence.
The Chlamydia Trachomatis FA Direct Test Kit uses fluorescein-conjugated murine monoclonal antibodies to detect extracellular elementary bodies in specimens taken directly from infected sites. The fluorescein-labeled monoclonal antibody is specific for the major outer membrane protein of both the elementary body and the reticulate body of C. trachomatis and will detect all 15 known serovars of this organism.
When the antibody conjugate is added to a slide containing the methanol-fixed specimen, the reagent will bind specifically to any C. trachomatis present in the smear. A washing step removes unbound antibody conjugate. When the stained slides are viewed under a fluorescence microscope, clinical specimens containing C. trachomatis show distinct apple-green elementary or reticulate bodies against a red counterstained background of cells.
The Chlamydia trachomatis Direct Specimen Kit supplies color coded dropper bottles containing Chlamydia Direct Reagent and Mounting Fluid (R62230) and Chlamydia control slides (R62205). Chlamydia Direct Reagent contains fluorescein-labeled purified murine monoclonal antibodies, Evans Blue counterstain, an inhibitor of nonspecific staining, and 0.098% sodium azide in a protein-stabilized buffer solution.
The Mounting Fluid contains buffered glycerol, with 0.01% sodium azide as preservative.
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