Gibco® Drosophila S2 cells are used for heterologous protein expression in the DrosophilaM Expression System (DES®). The S2 cell line was derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos. Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage. S2 cells grow at room temperature in ambient CO2 as a loose, semi-adherent monolayer in tissue culture flasks and in suspension in spinners and shake flasks. Drosophila S2 cells (frozen in Gibco® Schneider's Medium) feature: • Expression of protein transiently or through generation of stable cell lines • Quality and performance testing
Expression of protein transiently or through generation of stable cell lines Drosophila S2 cells can be transfected with the recombinant expression vector alone for transient expression studies or in combination with a selection vector (e.g. pCoHygro or pCoBlast) to generate stable cell lines. A variety of DES® kits are available to allow expression of the recombinant protein of interest in S2 cells.
Quality and performance testing Each lot of Gibco® S2 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.
Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.